油茶籽水酶法制水相固形物中茶皂素含量测定研究.doc免费

油茶籽水酶法制油水相固形物中茶皂素含量测定研究 郭咪咪 薛雅琳项目基金：公益性行业（粮食）科研专项 项目基金：公益性行业（粮食）科研专项：油茶籽加工链质量保障与提升关键技术研发（201513003-7） 收稿日期：2017-11-23 作者简介：郭咪咪，女，1987年出生，硕士，粮食、油脂与植物蛋白工程 通信作者：薛雅琳，女，1960年出生，教授级高级工程师，分析化学 （国家粮食局科学研究院 油脂化学研究组 北京 100037） 摘 要 本研究建立了一种液相色谱测定茶皂素含量的方法。色谱柱SUPELCOTM LC-18（4.6 mm*250 mm，5 μm）；流动相为甲醇-0.1%磷酸（体积比40:60）；流速0.8 mL/min；检测波长267 nm；柱温30℃。该方法平均加标回收率90.88%~113.13%，精密度达0.19%，定量检出限0.67%，测得水相固形物中茶皂素质量分数为54%，与比色法测定结果相接近，但液相法具有测定简便、快速、准确等特点；与相关液相测定茶皂素方法报道相比，该方法色谱峰分离较好，且避开了溶剂峰干扰等特点，适合作为测定茶皂素含量的分析方法。 关键词 水相固形物 茶皂素 含量 液相色谱 中图分类号：TS2 文献标志：A 文章编号： Quantitative Analysis of Saponin From Water phase solids produced in Camellia oil aqueous enzymatic extraction process Guo Mimi, Xue Yalin, Zhang Dong, Duan Zhangqun, Wang Yameng, Zhu Lin, Li Xiujuan, Li Xiaoning (Oil and Fat Chemistry Research Group, Academy of State Administration of Grain , Beijing 100037) Abstract A liquid chromatographic determination method of saponin content was established in this article. This method average recovery rate of saponin was 90.88%~113.13%, the degree of precision was 0.19%, the quantitative detection limit was 0.67%, and the mass?fraction of saponin from water phase solids was about 54%, when the determination conditions were as follows: SUPELCOTM LC-18 (4.6 mm*250 mm, 5 μm), mobile phase methanol-0.1% phosphoric acid solution ( volume ratio 40:60 ), flow rate 0.8 mL/min, detecting wavelength 267 nm, column temperature 30℃. Although the liquid chromatographic method result of saponin content was similar to the spectrophotometric method, this method was simplicity, quickness and accuracy. And this liquid chromatographic method had a better?separation?effect on chromatograph?peaks, and could avoid the solvent peak interference, when compared with the relevant report on liquid chromatographic method of saponin content. It?was?a suitable analyzes?method?for determination of saponin content. Key words water phase solids, saponin, content, liquid chromatographic 中国是