BENC511对人肺腺癌A549细胞增殖和苹凋亡的作用研究.docx

优秀毕业论文 精品参考文献资料 2． 2．BENC一511对A549细胞形态的影响 27 3，BENC一511对A549细胞周期的影响 ．．28 4．AnnexinV／PI双染法检测细胞凋亡率 ．29 5．BENC．51l处理后A549细胞线粒体膜电位的变化 ．30 6，BENC·51l对Cyclin A、PCNA和p2l蛋白表达水平的影响 31 7，BENC一511对Caspase一3、Caspase一9和P-Akt表达水平的影响 3l 讨论 ． ．33 结论 。38 参考文献 。39 致 谢 ． ．45 攻读硕士学位期间发表的论文 46 万方数据 ContentsChinese Contents Chinese abstract ． ． 一1 English abstract ． ．． ．． 4 symbolic description ． ． ．．．．．．．．．．． ．．．．．．．．．．． ．．．．．．．．．．． ．．．．．．．．．．．．-．- 8 Introduction ．． ．． ． ．9 1．Cell proliferation and apoptosis ··9 2．Lung cancer and A549 cells ．．1 0 3 target protein associated with lung cancer．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．1 1 3．1 PCNA．．．．．．．．．．． ．．．．．．．．．．． ．．．．． ．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．1 1 3．2 cyclin．．．．．．．．．．．． ．．．．．．．．．． ．． ． ． ． ．．．．．．．． ．．．．．．．．1 1 3．3 p2 1．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．． ．．．．．．．．．．． ．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．1 2 4．Small molecular compounds S14161 and BENC一51 1 f3 Materials and methods ． ． 1 5 1．Materials．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．1 5 1．1 Chemical compounds．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．15 1．2 Cell line ．．1 5 1．3 Reagents．．．．．． ．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．1 5 1．4 Reagent preparation．．．．．．．．．．．．． ．．．．． ．．． ．．．．．．．．．．． 1 6 1．5 Major instruments and equipments．．．．．．．．．．．．．． ．．．．．．．．．．． ．．．．．．．．．．1 8 2．Methods．． ． ． ．1 9 2．1 Cell cultrure ． 19 2．2 Effects of BENC．5 1 1 on the growth of A549 cells．．．．．．．．．．．．．． ．．．．．．．．20 2．2．1 MTT assay ． ． ． ． 20 2．2．2 Clone formation test． ．．．．．．．．．．． ．．．．．．．．．．． ．．．．．．． ． ．．．． ．．．．．．．．．20 2．3 Morphology change observed by light microscope．．．．．．．．．．．．． ．．．-．．．．21 2．4 cell cycle detected by flow cytometry 一21 2．5 Annexin V／PI double staining ． ．21 2．6 JC．1 ASS