《玉米芯的不同预处理对丙酮丁醇发酵的影响》-毕业论文（设计）.doc

PAGE 玉米芯的不同预处理对丙酮丁醇发酵的影响 摘 要 玉米芯是纤维素作物，其纤维素、半纤维素、木质素紧密结合在一起。经预处理后可以水解半纤维素和纤维素，增大水解效率，从而增大产物量。对于玉米芯分别用1%硫酸、10%氨水、0.3%磷酸和蒸馏水进行处理，再用其纤维素酶解液配制培养基，以丙酮丁醇梭菌ATCC824为菌种，进行厌氧发酵。比较不同处理方法对于丙酮丁醇发酵的影响。得到的结果是，硫酸预处理的玉米芯，其水解液发酵结果较好。丁醇产量为：0.30g/L。选定硫酸进行之后的正交试验，结果显示，水浴时间是4h，温度600C，而硫酸的浓度在这几个处理浓度之间影响不显著，所以只要以最低浓度(0.5～1.0%)处理即可。在以上条件下，丁醇的最高浓度为0.319 g/L。 关键词 玉米芯、预处理、丙酮丁醇发酵、硫酸 The effect of different pretreatment to the corncob on acetone-butanol fermentation Abstract Croncob belongs to cellulosic-based, and its cellulose, hemicellulose, lignose combine closely. Pretreating the corncob before hydrolysed can increase the efficiency of hydrolysis by hemicellulose and cellulose , and, increas the yield. The Corncob was preprocessed respectively with 1% sulfuric acid, 10% ammonia, 0.3% phosphoric acid and distilled water, and then the pretreatment corncob was hydrolysed by hemicellulose and cellulose. The enzymatic hyrolysate was used as culture for acetone-butanol fermentation. The bacteria was Clostridium acetobutylicum ATCC824. The result showed that using sulfuric acid to pretreat corncob, the concentration of the products was larger. The concentration of butanol was 0.30 g / L. Orthogonal test was conducted as corncob was pretreated with sulfuric acid. And the results showed that the better water bath time was 4 h, the better temperature was 60oC. And the effect of different concentration of sulfuric acid on fermentation was not significant, so the concentration of sulfuric acid was 0.5-1.0%. With the best pretreatment condition, the largest concentration of butanol was 0.319 g/L. Keywords Corncob, Pretreatment, Acetone-butanol fermentation, Sulfuric acid 目 录 TOC \o 1-3 \h \z \u 第1章 绪论 1 1.1 引言 1 1.2 丙酮丁醇发酵的意义 1 1.2.1 丙酮丁醇发酵中玉米芯预处理的必要性 1 1.2.2 玉米芯水解液的分步发酵[6] 3 第2章 材料与方法 4 2.1 实验材料、试剂、仪器 4 2.1.1 材料 4 2.1.2 试剂 4 2.1.3 仪器 4 2.2 实验方法 5 2.2.1 培养基、试剂配制 5 2.2.2 实验方法 8 第3章 结果与分析 13 3.1 还原糖标准曲线测定结果 13 3.2 玉米芯水分测定结果 13 3.3 纤维素酶活力测定结果 13