花生NAC转录因子的克隆及转录特征.docVIP

作物学报 ACTA AGRONOMICA SINICA 2009, 35(3: 541? 545 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ 本研究由国家自然科学基金项目 广东省自然科学基金 广东省教育厅自然科学重点项目 (C10033资助。 * 通讯作者 (Corresponding author: 李玲 , Tel: 020 E-mail: lilab@ 第一作者联系方式 : E-mail: liu5210500@163.com Received(收稿日期 : 2008-08-20; Accepted(接受日期 : 2008-10-06. DOI: 10.3724/SP.J.1006.2009.00541 花生 NAC 转录因子 AhNAC2和 AhNAC3的克隆及转录特征 刘 旭 李 玲 * 华南师范大学生命科学学院 / 广东省植物发育生物工程重点实验室 , 广东广州 510631 摘 要 : NAC 转录因子是特异存在于植物中具有多种生物功能的新型转录因子。 本实验从花生中克隆了 2个 NAC 基 因 AhNAC2和 AhNAC3(GenBank登录号为 EU755023和 EU755022; 蛋白序列分析表明 , 两者具有典型 NAC 转录因 子特征 , N端含有保守 NAC 结构域。半定量 RT-PCR 显示 , 在 ABA 和 GA 3处理 , 以及控水和低温条件下 , AhNAC2和 AhNAC3在花生组织中的表达上调 , 呈现不同的表达模式。 AhNAC2和 AhNAC3为典型的 NAC 转录因子新基因 , 蛋 白序列与拟南芥 RD26同源性较高 , 推测与响应干旱和 ABA 信号有关。 关键词 : NAC 转录因子; 基因表达调控; 信号转导 Cloning and Characterization of the NAC-Like Gene AhNAC2 and AhNAC3 in Peanut LIU Xu and LI Ling* Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China Abstract: NAC transcription factors existed differentially in plant are the new transcription regulatory factors with multiple bio-logical functions. Two NAC-like genes from peanut were cloned by RT-PCR and RACE methods, named AhNAC2 and AhNAC3 (GenBank accession No. EU755023 and EU755022, which contain an ORF of 1 050 bp and 1 008 bp and encode 349 and 335 amino acids, respectively. Gene sequence analysis showed that the putative protein of both two genes contained conserved NAC domain and highly different C terminal which is the typical characteristics of NAC transcription factors. Meanwhile, the transcrip-tion levels of the two genes were investigated by semi-quantitative RT-PCR, and the result showed that the expressions of Ah-NAC2 and AhNAC3 genes were enhanced by ABA, GA3, water stress, and cold stress respectively. Furthermore, the two genes expressed constitutively in peanut tissues, and their expression patterns were different i