喉癌EGFR和PCNA表达及DNA含量的研究.docVIP

???? 喉癌EGFR和PCNA表达及DNA含量的研究 ???? ???? 摘　要：目的：研究喉鳞癌中表皮生长因子受体（EGFR）、增殖细胞核抗原（PCNA）的表达和DNA含量，分析各指标间的相关性及与喉癌临床生物学行为和预后的关系。方法：用免疫组化SP法和像分析仪检测正常喉粘膜、喉鳞癌的EGFR、PCNA和DNA指数(DI)，结合临床资料进行分析。结果：喉鳞癌的EGFR的阳性表达率为54.8%,与正常喉组织的差异有统计学意义(P0.01)，但EGFR的阳性表达与喉癌的病理分级和5年生存率无显著相关。而PCNA阳性表达和DI则随肿瘤分化程度的降低而相应增高(P0.05)。两指标高者生存时间短，预后差(P0.05)。结论：EGFR的表达与喉癌的发生有一定关系，其过度表达可能为具有高度癌变危险的癌前病变提供早期诊断的辅助指标。肿瘤恶性程度的判断和预后评价则以检测PCNA的同时配以DNA含量测定更有价值。关键词：喉肿瘤　鳞状细胞癌　表皮生长因子受体　增殖细胞核抗原　DNA含量 Expression of EGFR and PCNA, and DNA content in squamous cell carcinoma of larynx LING Ling　ZHOU Min-yu（ Department of Otolaryngology , First Affiliated Hospital, Zhejiang Medical University, Hangzhou 310003）WANG Hui-e（Department of Otolaryngology,Second Affiliated Hospital,Zhejiang Medical University） Abstract：Objective：To investigate the expression of epidermal growth factor (EGF), proliferating cell nuclear antigen (PCNA) and DNA index (DI) in laryngeal carcinoma,to analyse the correlation between these index and the biological characteristics of laryngeal carcinoma and their values of clinical prognosis. Method： Immunohistochemical staining was used to detect the expression of EGFR and PCNA in laryngeal cancer and normal tissue, and with MIPS-I image analysis system DNA contents of cancer cell were measured and made out DNA index. Result：The positive rate of EGFR in laryngeal carcinoma was 54.8%, and it was negative in all 10 normal laryngeal mucosa specimens (P 0.01). The expression of EGFR did not correlate with histological grading and 5-years survial rate (P ＞0.05), The positive expression of PCNA and DNA contents in the laryngeal carcinoma were increased with the decrease of tumorous differentiation (P 0.05). With the increasing of PCNA positive expression and DI, the prognosis of the patients were poorer (P 0.05). Conclusion：EGFR may be related to the process of carcinogensis in laryngeal carcinoma and was used as an early biomarker identifying premalignant lesions which had the greatest risk of carcingesis. PCNA an