p53参与缺血后处理对脊髓原代培养神经元缺血-再灌注损伤保护作用的机制研究.pdf

[1]   Yang T,et al.NEUROLOGIA MEDICO­CHIRURGICA, 2015. [2]   Zhu P,et al.MEDIATORS OF INFLAMMATION, 2013. POST:   time OGD + time reperfusion Xie,et al. J Neurochem. 2013 Xie, et al. Stroke. 2014 Xie, et al. Brain Res. 2015 I/R  injury Post I/R  conditioning injury p53  p53 targets (BAX…) p53 S6K1 Protein  Apoptosis synthesis 1. (Werner et al. Neuron.1989; Xie et al. Stroke. 2014) 2. OGD and Postcondtioning model (Xie et al. Brain Res. 2012; Xie et al. Stroke. 2014) 3. (Xie et al. JCBFM. 2013; Xie et al. Stroke. 2014 4. p53 MDM2-shRNA 9 addgene 1x10 /μl (Xie et al. Stroke. 2014; Xie et al. Brain Res. 2015) 5. Western blotting (Xie et al. JCBFM. 2013; Xie et al. Stroke. 2014 6. Statistic SPSS 16.0, Fisher test MAP2 DAPI Merge Reperfusion time + OGD  time ** p0.01 vs. No-OGD # p0.05 vs. No-OGD ## p0.01 vs. No-OGD p53 actin OGD - + +