13王金凯--RNA splicing 生物信息学PPT技术文档.pptxVIP

Transcriptomics--RNA splicing王金凯中山医学院A piece of human genomeSplicingAlternative splicingVariants of RNAHisatData visualizationUCSC genome browser. ()IGV tool/software/igv//software/igv/Wig and bigwig formatgenomeCoverageBed -bg -ibam –split sample.sorted.bam –g chromInfo.txt sample.bedgraph (bedtools /projects/bedtools//projects/bedtools/ )bedGraphToBigWig sample.bedgraph ~/chromInfo.txt sample.bw (/admin/exe//admin/exe/ )Wig:RNA-Seq Data for Alternative Splicing AnalysesInclusion ReadsNotation:: Exon inclusion level, the percentage of isoforms including the exon.: Read counts of inclusion, s