培养条件对阴沟肠杆菌乐果降解效能的影响.docVIP

培养条件对阴沟肠杆菌乐果降解效能的影响 培养条件对阴沟肠杆菌乐果降解效能的影响 官雪芳1, 2,刘波1*,林斌2,林抗美1,马丽娜1 (1.福建省农业科学院生物资源研究所，福建福州350003； 2.福建省农科院农业工程技术研究所，福建福州350003) 摘要：从福建省福农生化有限公司排污口的土壤屮筛选到一?株对乐果有较好降解效果 的菌株阴沟肠杆菌，为了使该菌能更好的应川于乐果残留污染的治理当屮，特采川正交试 验法L16(44X2：3)研究各培养条件对该菌降解乐果的降解效能的影响，结果为：该菌降 解乐果的最佳发酵条件组合为处理5,具体为碳源：蔗糖、离子：CuC12:(10 Mmol?L- 1)、乐果浓度：100 mg?L-l、培养时间36 h、培养温度30 °C、pH值8、氮源：酵母3 g?L-l,降解率达到58.90%,运川DPS数据处理系统分析各类因子对菌降解乐果的彩响， 得出离子对其作用影响最为显著，pH值、培养温度、乐果浓度、培养时间等也依次对菌降 解乐果有较犬的影响。 关键词：阴沟肠杆菌乐果 降解 优化 止交试验 屮图分类号：X172文献标识码：A Effect of culture conditionson Enterobacter closcae degrading dimethoate Effect of culture conditions GUAN Xue-fangl, 2, LIU Bol*丄IN Bin2, LIN Kang-meil, MA Linal (1 Agricultural Bioresources Research Institute Fujian Academy of Agricultural Sciences, Fuzhou,350003, China, 2Institute of Agricultural Enginecring Technology; Fujian Academy of Agricultural Sciences, Fuzhou,350003, China ) Abstrat: By using selective cultural media, Enterobacter closcae was isolated to degrade dimethoate from the soil around the pesticide factory. In order to make it better used with residual dimethoate, the L16 (44*23) orthogonal test was app1icated to study the effective factors of thedegradation to dimethoate of E. closcae. The results showed that the conformable carbon source for the degradation was sucrose, the ion was Cu2+ with the density of 10 Mmol/L, optimum temperature was 30 °C,the density of dimethoate was 100 mg/L, thethe initial pH was 8. 0, the conformable with the density of 10 Mmol/L, optimum temperature was 30 °C, nitrogen source was yeast extract with thedensity of 3 g/L,and the culture nitrogen source was yeast extract with the density of 3 g/L, and the culture Meanwhile, using the DPStime was for 36 h with the degrading rates of 58.90%, Meanwhile, using the DPS Date Processing System, the single factor effect and sample were statisticed. The effect of ion has 收稿日期:2009-2-12 初稿;2009-修改稿 作者简介：官雪芳（1979-）,女，硕士，研究实习员，主要从事环境微生物方面的研 究。E-ma订:guan-619@163p *通讯作者:刘波（1957-）,男,博士，研究员,主要从事 微生物生物技术和农