dectin-1和dectin-2在抗真菌免疫中的作用.docxVIP

226 · 国际呼吸杂志 2013年 2月第 33卷第 3期 IntJRespir，February2013，Vo1．33，No．3 综述 ． Dectin一1和 Dectin一2在抗真菌免疫中的作用 孙禾 徐小勇 苏欣 施毅 【摘要】 Dectin-1和 Dectin-2是 Ⅱ型跨膜蛋 白，属 c型凝集 素受体家族，在胞外区有独立的糖结合域，主要在树突状细胞和巨噬细胞表面表达。Dectin-1识别 真菌细胞壁上的 B一葡 聚糖，依赖胞浆内的酪氨酸激活基序(ITAM 样基序)传递信号；Dectin～2识别 一甘露聚糖，通过胞内的补体 FC段 的 链结合 1TAM 样基序传递信号。激活后的 Dectin一1和 Dectin-2募集脾脏酪氨酸激酶与酪氨酸激活基序 (ITAM 基序 )结合，并激活 CARD9一NF一B信号通路 ，导致促炎因子等多种基因表达。Dectin一1诱导真 菌感染宿主产生活性氧，在免疫防御中发挥重要作用。Dectin一1和 Dectin 2均优先诱导 Thl7细胞 分化，对真菌感染宿主起保护作用。 【关键词】 Dectin]；Dectin-2；细胞因子；固有免疫；Thl7 Dectin-1 and Dectin一2 in innateimmunity againstfungalinfection SUN H e，XU Xiao yong，SU Xin，SH I Yi．Department of Respiratory and Critical Care Medicine，Jinling Hospital，Nanjing Clinical College，Second M ilitary MedicalUniversity，Nanjing 210002，China C0rr 声。 d g author：SH IYi，Email：shiyi56@ 126．cD”2 [Abstract] Dectin-1 and Dectin一2 are type I transmembraneproteinsofthe C—type lectin family with single carbohydrate recognition domains (CRD ) in the extracellular region，which are mainly expressed in dendritic cells and macrophages． Dectin一1 recognizes beta glucans with its CRD and transduces signals through its imm unoreceptor tyrosine—based activation motif(ITAM )一like motifin the cytoplasmic domain， while Dectin-2 recognizes alpha—mannans and transduces its signal through association with the ITAM—containing Fc receptor gamma chain． Upon ligand binding，spleen tyrosine kinase isrecruited tO the ITAM and activatesthecaspase recruitmentdom ain fam ily member9 (CARD9)一 nuclearfactor-kappa B axis， resulting in the activation of various genes including those encoding pro— inflammatory cytokines，Dectin-1 is im portantin protection againstfungalinfection by inducing reactive oxygen species， whereas both Dectin-1 and Dectin 2 play important roles in defense against fungi by preferentialy inducing T(h)17 celdifferentiati0n． [Keywords] Dectin-1；Dectin一2；Cytokine；Innateimmunity；Thl7 随着对侵袭性真菌感染机制研究的深入，配体一受体间的关系及其信号转导机制日益引起关注。近期研究证实，模式识别受体(PRRs)在宿主体内分布广泛，参与识别各种病原相关分子模式 (PAMPs)及体内损伤相关