fenton氧化_微生物法降解土壤中石油烃_韩旭.docxVIP

化 工 环 保 2017年第 37卷第 2期 ENVIRONMENTAL PROTECTION OF CHEMICAL INDUSTRY ·237· 土壤修复 Fenton氧化—微生物法降解土壤中石油烃 韩 旭，李广云，尹宁宁，许锐伟，王丽萍 （中国矿业大学 环境与测绘学院，江苏 徐州 221116） ［摘要］ 以长期被苯系物污染的活性污泥为菌源，采用液相“诱导物-中间产物-目标污染物”驯化模式驯化出专性混合石油降解菌群，并将其用于Fenton氧化—微生物法处理模拟石油污染土壤。高通量测序结果表明，产黄杆菌属（Rhodanobacter）、分支杆菌属（Mycobacterium）和根瘤菌属（Rhizobiales）为主导菌属。实验结果表明：接种混合菌群后降解50 d，土样的总石油烃（TPH）去除率较土著菌提高了13.4~20.5百分点；对于TPH含量（w）分别为4%，8%，11%的土样，Fenton氧化的最佳H2O2加入量分别为3，4，4 mol/L（Fe2+加入量0.04 mol/L），TPH总去除率分别可达88.8%，65.0%，47.7%，较单独Fenton氧化或单独微生物法均有很大程度的提高，且缩短了降解时间，增加了土壤有机质。 ［关键词］ 专性混合石油降解菌；Fenton氧化；微生物法；石油烃 ［中图分类号］ X53 ［文献标志码］ A ［文章编号］ 1006-1878（2017）02-0237-06 ［DOI］ 10.3969/j.issn.1006-1878.2017.02.020 Degradation of petroleum hydrocarbons in soil by Fenton oxidation-microbial method Han Xu，Li Guangyun，Yin Ningning，Xu Ruiwei，Wang Liping （School of Environment Science and Spatial Informatics，China University of Ming and Technology， Xuzhou Jiangsu 221116，China） Abstract：Using the activated sludge which was long-term polluted by benzene as bacteria source，the specific mixed petroleum-degrading bacteria were acclimated in liquid phase by the model of “inducer-intermediate product-target pollutant”，and used for treatment of simulated petroleum contaminated soil by Fenton oxidation-microbial method. The high-throughput sequencing results showed that Rhodanobacter sp.，Mycobacterium sp. and Rhizobiales sp. were the dominant bacteria. The experimental results indicated that：After 50 d of degradation，the removal rate of total petroleum hydrocarbon（TPH）in the soil samples by mixed bacteria was 13.4-20.5 percentage points higher than that by native bacteria；When the TPH content（w）were 4%，8%，11%，the optimum H2O2 concentration for Fenton oxidation were 3，4，4 mol/L respectively（with 0.04 mol/L of Fe2+ concentration），the total removal rate of TPH were 88.8%，65.0%，47.7% respectively，which were much higher than those by single Fenton oxidation or single microbial method. The degradation time was shortened and organic matters in soil we