第四章细菌的遗传分析.pptxVIP

第四章? 细菌的遗传分析;第一节 细菌的接合（Bacterial conjugation);第四章细菌的遗传分析;;Plasmids: smaller cycles of double-strand DNA that do not carry essential genes;F plasmid (sex factor, fertility factor) ;第四章细菌的遗传分析;第四章细菌的遗传分析;;;第四章细菌的遗传分析;第四章细菌的遗传分析;;第四章细菌的遗传分析;Because the transfer is usually interrupted in the midstream, the second half of the F plasmid is not transferred. Only about 1/10000 Hfr cells transfers an entire strand of its chromosome to a recipient F－ cell ;;;Formation of an F’ plasmid;merozygote;Using F’ plasmid for complementation analysis;;第四章细菌的遗传分析;第二节 中断杂交实验与重组作图Interrupted mating experiment and recombination mapping;1. Interrupted mating experiment ;;第四章细菌的遗传分析;Comparisons of interrupted mating studies using different Hfr strains confirm that the bacterial chromosome is a circle;2. Recombination mapping;◇ Property of bacterial recombination;;◇ Recombination mapping;Donor: lac＋ade ＋ Recipient: lac－ade－;第四章细菌的遗传分析;3. The relationship of interrupted mating mapping and recombination mapping;第三节 转化与转化作图Transformation and mapping;1. Transformation process;Proposed steps leading to transformation;第四章细菌的遗传分析;;第四章细菌的遗传分析;2. Transformation mapping;locus ;第四节 转导与转导作图Transduction and mapping ;;;◇注入的细菌染色体整合进宿主染色体上. 也可能游离于细胞质中，称为流产转导（abortive transduction） ◇常用的普遍性转导噬菌体为E.coil P1噬菌体;;;;;第四章细菌的遗传分析;An overview: Three mechanisms of gene transfer in bacteria