龟鹿补肾丸主要成分的检验方法的研究.docVIP

龟鹿补肾丸主要成分的检验方法的研究 摘要：本文研究了龟鹿补肾丸的主要成分的定性定量检验的方法。采用显微镜进行观察，同时采用TLC法对龟鹿补肾丸进行定性鉴别；使用HPLC法测定该制剂中淫羊藿苷的含量。结果：薄层色谱法用0.5％NaOH溶液制备的硅胶G板，以甲苯—乙酸乙酯—甲酸（15:2:1）以展开剂，365nm紫外光灯照射的条件下可鉴别出该制剂中的何首乌；用硅胶G板，以三氯甲烷—甲醇—水（13:7:2）的下层溶液为展开剂，以10%硫酸乙醇溶液为显色剂，105℃加热的条件下能鉴别出黄芪；用硅胶G板，以甲醇—丁醇—三氯甲烷—水（4:6:6:1）为展开剂，以5%三氯化铝乙醇溶液为显色剂，105℃加热，紫外光灯365nm照射下可鉴别出淫羊藿；用硅胶G板，甲苯—乙酸乙酯—甲酸—水（20：10:1:1）的上层溶液为展开剂，展至约15cm，以5%三氯化铝乙醇溶液为显色剂，365nm紫外光灯照射下可鉴别出陈皮。HPLC法含量测定中，淫羊藿苷的浓度在1～30μg·mL-1范围内呈良好线性，样品的平均加样回收率为99.87%。 关键词：龟鹿补肾丸；显微鉴别；薄层色谱法；高效液相色谱法 Study on Testing Methods for the Main Components of Guilu Bushen Pills [Abstract] This article focuses on the qualitative and quantitative test methods from the main component of the guilu bushen pills. Guilu bushen pills are treated with qualitative identification by microscopic identification method and TLC; The lcariin of the pills was determined by HPLC. Results: When TLC method is applied, Silica gel G plate prepared by 0.5%NaOH solution used as the stationary phase , toluene - ethyl acetate - formic acid (mole ratio is 15:2:1) used as the developing agent, polygonum multiflorum is identified at the wavelength of 365 nm; When silica gel G plate is used as the stationary phase , chloroform - methanol - water (13:7:2) solution used as as the developing agent, 10% sulfuric acid in ethanol used as chromogenic reagent, heated to 105℃, astragalus is identified at the wavelength of 365 nm; When silica gel G plates used as the stationary phase, methanol - butanol - chloroform - water (mole ratio is 4:6:6:1) as the developing agent, 5% ethanol solution of aluminum chloride as chromogenic reagent, heated to 105℃, epimedium is identified at the wavelength of 365 nm ; When silica gel G plate used as the stationary phase ,the upper solutionof toluene - ethyl acetate - formic acid - water (mole ratio is 20:10:1:1) used as developing solvent, 5% ethanol solution of aluminum chloride as chromogenic reagent, dried tangerine peel can be identified at the wavelength of 365 nm. HPLC method for