中国肉盘衣属地衣新记录种.docxVIP

中国肉盘衣属地衣新记录种 The genus China had previously reported nine1 Materials and methods1.1 Specimens and morphologyThe specimens of new record are deposited in the Fungarium of College of Life Sciences, Liaocheng University (LCUF) and Herbarium Mycologicum Academiae Sinicae-Lichenes (HMAS-L). A dissecting microscope (Olympus SZX16) and a light microscope (Olympus BX53) were used for the morphological and anatomical studies. Measurements were taken from mature vertical sections of fruit bodies mounted in water. 1.2 ChemistryAmyloidity of the ascospores was tested using Lugol’s solution. Spot tests with K (10% aqueous solution of potassium hydroxide), C (saturated solution of aqueous sodium hypochlorite), and P (saturated solution of p-phenylenediamine in 95% ethyl alcohol) were performed on the thallus surface. The lichen substances were detected and identified by thin-layer chromatography, using solvent C1.3 DNA extraction, amplification, and sequencingGenomic DNA was extracted from ascomata of the specimens using the Hi-DNAsecure Plant Kit (Tiangen, Beijing, China) according to the manufacturer’s protocol. PCR amplification was performed using the mtSSU1 and mtSSU3R primer pair for mtSSU1.4 Phylogenetic analysis Contigs were assembled and edited using the program Geneious v6.1.2 (Biomatters Ltd., Auckland, NZ). A total of 39 sequences were aligned using MAFFT v.72 Results2.1 Phylogenetic analysisThe final alignment consisted of 9 newly generated mtSSU sequences and 30 sequences downloaded from NCBI (Fig. 1). The phylogenetic trees obtained from maximum likelihood (ML) and Bayesian inference analysis (BI) exhibited the same topology; we therefore present only the ML tree. The molecular phylogeny based on the mitochondrial small subunit marker (mtSSU) of Pilocarpaceae exhibits a well-supported monophyletic lineage containing the genera 2.2 Taxonomy (Vain.) Lücking Sérus, in Lichenologist 33(3): 192 (2001) Fig. 2≡ ≡ Type: Philippines, Ro