分子荧光法分别测定4种野生可食植物中的色氨酸和酪氨酸_高向阳.pdfVIP

2012, Vol.33, No.22 231 4 1 1 2 2 (1. 450002 2. 450064) 6mol/L 1.372.63mg/g7.9825.83mg/g 5.4% 0.011μg/mL 0.037μg/mL 93.5%105% 0.033μg/mL0.11μg/mL94.6%100.5% Determination of Tryptophan and Tyrosine in Four Edible Wild Plants by Molecular Fluorescence Spectrometry 1 1 2 2 GAO Xiang-yang ZHANG Kai-ge KONG Xin-xin YOU Xin-xia (1. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China 2. Department of Food Science, Zhengzhou Institute of Science and Technology, Zhengzhou 450064, China) Abstract A new method was established to determine tryptophan and tyrosine in four edible plants wildly grown in Zhengzhou region, Sonchus asper, Plantago asiatica, Vigna minima and Pyracantha fortuneana . Samples were hydrolyzed with with 6 mol/L sodium hydroxide solution determined by molecular fluorescence spectrometry. The average contents of tryptophan and tyrosine in the wild plants were 1.37–2.63 mg/g and 7.98–25.83 mg/g, respectively, with a relative standard deviation equal to or less than 5.4% (n = 5). The proposed tryptophan standard curve showed good linear relationship over the concentration range of 0.00–0.10 μg/mL. The method exhibited a limit of detection of 0.011 μg/mL and 0.033 μg/mL and a limit of quantification of 0.037 μg/mL and 0.11 μg/mL for tryptophan and tyrosine, respectively. The propose tyrosine standard curve displayed a linear range of 0.0–1.0 μg/mL. The average spike recovery rates of tryptophan and tyrosine in these four plants were 93.5%–105% and 94.6%–100.5%, respectively. This method was sim