不同方法提取绿原酸的比较研究.docVIP

PAGE PAGE 28 摘要 绿原酸是金银花的主要抗菌、抗病毒有效药理成分之一。本文对金银花中提取绿原酸的不同方法进行了研究，分别采用了煎煮法，乙醇回流法和微波辅助法三种方法进行提取。得出产物后用紫外分光光度计进行吸光度的测定，和标准曲线进行对比，从而计算出提取率。在煎煮法中，分别采用料液比、煎煮温度和煎煮时间为变量，得出最优条件为料液比1:15，煎煮时间60min，温度70℃；乙醇回流法中，分别采用料液比、回流时间和乙醇浓度作为变量，控制回流温度在70℃，得出最优条件为料液比1：15，乙醇浓度70%，回流时间2h；微波辅助法中，分别采用微波时间、微波功率和溶液PH值为变量，原料进行预处理，浸渍24h，控制回流温度为 关键词：绿原酸金银花煎煮法乙醇回流法微波辅助法 Abstract Chlorogenic acid is the main anti-microbial and antiviral pharmacological component in honeysuckle. My paper has studied three ways to extract chlorogenic acid from honeysuckle and find the best way. The first way is water-bath heating extraction with three factors which are water-bathing time, temperature and ratio of solid to liquid. The best condition goes with 60min extracting time, 70 degrees and 1:15 ratio of solid to liquid. The second method is ethanol reflux extraction also with three factors which are ethanol concentration, reflux time and ratio of solid to liquid. The reflux temperature is around 70 degrees. The best condition goes with 70% ethanol concentration 2h reflux time and 1:15 ratio of solid to liquid. The last method is Microwave Aided Extraction. The factors are power, time and solution’s PH. The materials need to be dipped for 24 hours before experiment. And the reflux temperature is controlled around 70 degrees and reflux time is 30min. The best condition of this method goes with 15min microwave time, 300W microwave power and PH=5. Compared with other two methods, microwave aided extraction has the highest extraction efficiency. Keywords: Chlorogenic acid; water-bath heating extraction; ethanol reflux extraction; microwave aided extraction. 1 绪论 1.1 金银花的概况 1.1.1金银花的简介 金银花，学名忍冬。总所周知，忍冬作为一种常用中药，有着久远的历史，始载于《名医别录》，列为上品[1]。在秦汉时期，著名中药专著《神农本草集》曾有记载就载有关于金银花的学名，称其“凌冬不凋”。金银花的名称在《本草纲目》中也有详细的讲解，里面提到金银花，又名金银藤、鸳鸯藤、鹭鸶藤等。处处有之，藤生，凌冬不雕，故名忍冬。 [2] 金银花的枝干很细，中间是空的，藤条大多为咖啡色，有的呈红褐色。开花时间一般为6月到8、9月的夏季，苞片叶状，花瓣似唇型，带有微微香气，外面有柔毛和腺毛，雄蕊和花柱均伸出花冠，花色刚开始为白色，接着逐渐变成金黄，黄白相互交映，球形浆果，成熟时多显黑色[3]。 图1.1 金银花 金银花有对环境容忍性较强，喜阳、不仅在阴暗处、寒冷地带能生长，也可生存