生物工程学中新型的应用合成生物学的体外代谢途径.pptVIP

生物工程学中新型 应用合成生物学 体外代谢路径;关键点;摘要;介绍;Figure1.?Complex molecules and pathways as targets for synthetic biology. (a) Arixtra, (b) A premier example of the synthetic power of systems of enzymes (adapted from [9]): the formation of ketose-1-phosphate compounds from sucrose in an eight-enzyme system. ; 不过, 尽管有了很好 基于酶 系统, 化学路径在工业中仍然是 占关键位置 。现在酶系统在工业中极少应用 原因: 1.极难比较经济 组合一个酶系统 2.因为底物、中间物和产物与酶 相互作用使得这个系统 动态行为 很复杂, 不好了解。另外, 系统倾向于朝着细胞稳态, 而不是提升生 产力所要求 非自然状态 方向发展 3.在试验中促进第二点 方法非常猛烈 尽管如此, 这种多酶系统毕竟在大小和复杂性上被限制了（尤其 是对于整个细胞系统）, 另外, 我们搜集酶系统 能力一直在加强 , 分析方法 使用范围和潜力也一直在扩大, 所以我们最少能够半合 理性 设计酶 功效特征。最终, 拥有合理设计所需要 全部条件。;;多酶系统 组合;数据 产生;Figure 3.?The main steps involved in quantitative MS-based analysis of intracellular metabolites and typical time ranges required for individual steps. Continuous direct injection has so far only been used for qualitative purposes, not quantitative, and requires MS/MS technology. ;酶系统模型;网络 数学优化;网络 实际优化;Figure 4.?The kinetic properties of enzyme n as an element in a system of enzymes need to be adapted to increase the overall efficiency of the system. The example enzyme is uncompetitively inhibited by substrate n+2 .By using either directed evolution or a (semi-)rational approach that drastically limits the required number of mutants to screen, the required adaptation is implemented in an iterative approach.;结论 ;