独活寄生汤对兔膝骨性关节炎关节液白细胞介素1和肿瘤坏死因子的影响.docVIP

独活寄生汤对兔膝骨性关节炎关节液白细胞介素1和肿瘤坏死因子的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：独活寄生汤对兔膝骨性关节炎关节液白细胞介素1和肿瘤坏死因子的影响 1 1 材料与方法 3 2 结 果 4 3 讨 论 4 文2：铺灸对佐剂型关节炎大鼠白细胞介素1和肿瘤坏死因子α白细胞介素10的影响 6 1 实验材料 7 2 实验方法 7 3 指标测定 8 4 统计学方法 9 5 结果 9 6 讨论 9 参考文摘引言： 10 原创性声明（模板） 12 文章致谢（模板） 12 正文 独活寄生汤对兔膝骨性关节炎关节液白细胞介素1和肿瘤坏死因子的影响 文1：独活寄生汤对兔膝骨性关节炎关节液白细胞介素1和肿瘤坏死因子的影响 【Abstract】Objective To observe the effect of Duhuojisheng decoction on the expression of IL-1 and TNF from synovial fluid in rabbit with osteoarthritis. Methods 24 rabbits were randomly divided into control and treatment groups. There were 12 rabbits (24 articular genus) in every group. Osteoarthritis model was established by injection of caroid in articular genu intracavitary. Rabbits with osteoarthritis were interfered by Duhuojisheng decoction. The expression of IL-1 and TNF from synovial fluid in rabbit with osteoarthritis were measured by double antibody sandwich ELISA 1, 3, 5 weeks after treatment. Results There were expression of IL-1 and TNF from synovial fluid a week after treatment. Following weeks expression of IL-1 and TNF raised in control group. In 3 weeks peak appeared. Expression of IL-1 and TNF raised in treatment group decreased compared with those in control group (P, P). Conclusion Duhuojisheng decoction can depress the expression of IL-1 and TNF in osteoarthritis. 【Key words】Osteoarthritis; Duhuojisheng decoction; Interleukin-1; Tumor necrosis factor; Rabbit 骨关节炎是骨伤科临床常见病、多发病，主要表现为关节疼痛、僵硬及功能障碍，其主要病理特征是关节软骨退变及相应炎性改变，发病较为缓慢，是一种严重影响老年人健康的疾病。目前，常用的治疗方法有中西医之分，西医以服用非甾体消炎药消炎止痛缓解症状为主，中医中药则活血化瘀、通经活络辨证施治。中医学认为，长期劳损、血瘀气滞、风寒湿邪、痹阻经络、肾虚等因素致使骨髓空虚，血气亏损，筋骨失其所养，易于损伤，感受外邪而发为痹，故骨性关节炎为痹证，可通过补肾通络法治疗。独活寄生汤具有祛风湿、止痹痛、益肝肾、补气血的功效，常用于治疗风湿痹痛等病症，对骨性关节炎有较好的治疗作用。近年来研究发现骨性关节炎与众多炎性因子有关，其中白细胞介素( IL-1) 及肿瘤坏死因子(TNF)是参与骨关节炎进程的重要介质。我们通过观察兔膝关节液中IL-1 及TNF在独活寄生汤治疗前后的变化，探讨独活寄生汤治疗骨性关节炎的机制。 1 材料与方法 实验动物及分组 4～6个月龄健康大耳白兔24只，体质量～ kg，雄雌不限,华北煤炭医学院动物中心提供，合格证号:2006-04。随机分为对照组、治疗组，各12 只。 造模方法 按照日本头康彦等［1］的兔膝关节腔内注入木瓜蛋白酶，造成骨