高效液相色谱法测定双黄连粉针剂中绿原酸和黄芩苷的含量.docVIP

高效液相色谱法测定双黄连粉针剂中绿原酸和黄芩苷的含量 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：高效液相色谱法测定双黄连粉针剂中绿原酸和黄芩苷的含量 1 1 器材 2 2 方法与结果 3 3 讨论 7 文2：高效液相色谱法测定天山花楸中的绿原酸含量 7 1 仪器与试药 8 2 方法与结果 9 3 讨论 11 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 13 正文 高效液相色谱法测定双黄连粉针剂中绿原酸和黄芩苷的含量 文1：高效液相色谱法测定双黄连粉针剂中绿原酸和黄芩苷的含量 Quantitative Determination of Chlorogenic Acid and Baicalin in Shuanghuanglian Powder Injection by HPLC Abstract：ObjectiveTo develop an RP-HPLC method for the determination of chlorogenic acid and baicalin in Shuanghuanglian powder C8~C13mixedalkyl (5μm， mm×150 mm)bond silica for reveed-phase HPLC was prepared. The mobile phase was CH3OHphosphoric acid solution (pH ). The flow rate was set at ml/min. The detection wavelength was at 323 nm. Chlorogenic acid and baicalin were separated by HPLC with grade elution. ResultsThe linearity ranges of chlorogenic acid and baicalin were ~ mg /ml and ~ mg /ml respectively，the correlation coefficients were 89 and 97 ,the average recoveries of adding sample were % and %, detection limits were mg/ml and mg /ml, the D (n=5) of measurement precision test were % and %, the D (n=5) of reproducibility between tests were % and %. The content of chlorogenic acid in Shuanghuanglian powder was ~ mg/ml，and that of baicalin was ~ mg/ml. ConclusionThe method is simple, fast, accurate，seitive, reproducible and low cost. It is fit for the quality control of Shuanghuanglian powder. Key words：Shuanghuanglian powder; Chlorogenic acid; Baicalin； HPLC 双黄连粉针剂是供注射用的中药制剂，由金银花、黄芩和连翘组成，具有清热解毒、辛凉解表的功能。主要成分为绿原酸、黄芩苷，质量控制多采用黄芩苷和绿原酸作为指标。含量测定方法有紫外分光光度法［1，2］、高效毛细管电泳［3，4］及高效液相色谱法［5~9］等。由于该制剂成分中黄芩苷和绿原酸极性相差较大,故质量标准中规定分别测定二者含量。本实验采用自制C8~C13混合型烷基键合硅胶柱［10］，利用梯度洗脱，逐渐降低流动相极性的方法不经分离同时测定四批样品中绿原酸和黄芩苷的含量，以期为该制剂的含量的质量控制提供 参考 。 1 器材 仪器KNAUER高效液相色谱仪（德国）由K-501HPLC泵、分析型动态混合器、K-2600UV Detector、7725i手动进样阀、Eerochrom 2000 Basic Edition 色谱工作站软件 和T3000柱温箱（北京创新通恒科技有限公司）组成。KQ-400BD型数控超声波清洗器（昆山市超声仪器有限公司），C8~C13混合型烷基键合硅胶色谱柱（自制），PHS-3C精密pH计（上海雷磁仪器厂），TU-1900光度扫描仪。 药品与试剂双黄连粉针剂（哈尔滨医药股份有限