熊胆冻干粉针剂对缺氧.docVIP

熊胆冻干粉针剂对缺氧 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：熊胆冻干粉针剂对缺氧 1 1 材料与方法 2 2 结果 4 3 讨论 5 文2：熊胆冻干粉针剂安全性实验报告 7 1 药物 8 2 方法 8 3 结论 11 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 熊胆冻干粉针剂对缺氧 文1：熊胆冻干粉针剂对缺氧 Protective Effects of the Lyophiled Powder Injection of Bear Bile on Vascular Endothelial Cells Induced by Hypoxia-reoxygenation Injury Abstract：ObjectiveTo study the protective effects of the lyophiled powder injection of bear bile on cultured vascular epithelial cells (VEC) induced by hypoxia-reoxygenation injury, and study the mechanisms of anti -atherosclerosis. MethodsBy using primary culture of human umbilical vein endothelial cells (HUVEC) in vitro, the VECs were divided into three morphological changes of VEC by the inverted microscope, the optical deity (OD) value of cells were detected by MTT colorimetric method, content of MDA and NO in the supernatant fluid of cultured cells were detected by MTT assay showed that OD value had significant difference (P) between normal group, bear bile group and hypoxia-reoxygenation group. The MDA content increased and NO content decreased in the hypoxia-reoxygenation group, in contrast, MDA content decreased and NO content increased in the normal and bear bile groups. ConclusionThe results indicate that the lyophiled powder injection of bear bile has protective effects for the HUVEC injury induced by hypoxiareoxygenation injury . Key words：Bear bile; Vascular epithelial cell; Malondiadehyde; Nitric oxide 缺血-再灌注是再灌注心肌损伤、缺血性脑卒中等严重疾病中常见的过程。缺血缺氧极易导致血管内皮细胞(vascular endothelial cell，VEC)的死亡和严重功能紊乱，从而进一步加重组织损伤。目前关于缺血-再灌注时细胞损伤的机制及对其的保护的研究主要集中在神经细胞和心肌细胞，对VEC的研究极少。本实验通过用连二亚硫酸钠造成缺氧－再复氧模型后用熊胆，观察其抗缺氧作用及其机制。 1 材料与方法 材料 人脐静脉内皮细胞 人脐静脉内皮细胞（human umbilical vein endothelial cell，HUVEC），以酶消化法取得。 试剂与主要仪器 熊胆冻干粉针剂由延边大学药学院提供。人第八因子相关抗原（Ⅷ因子）是美国 Santa cruz 产品，一氧化氮（NO）和丙二醛（MDA）检测试剂盒购置于南京建成生物有限公司。M199培养基为美国GIBCO公司产品；胎牛血清为美国HYCOLONE公司产品；连二亚硫酸钠为沈阳市东兴试剂；MTT为SIGMA公司产品。倒置相差显微镜，日本OLYMPUS产品，37度CO2培养箱是德国SL产品。 方法 脐静脉内皮细胞的分离及培养 按Jaff