青蒿琥酯对佐剂性关节炎滑膜组织中凋亡因子FasFasL及Bcl.docVIP

青蒿琥酯对佐剂性关节炎滑膜组织中凋亡因子FasFasL及Bcl 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：青蒿琥酯对佐剂性关节炎滑膜组织中凋亡因子FasFasL及Bcl 1 1 材料与方法 2 2 结果 4 3 讨论 6 文2：气虚血瘀证大鼠血管平滑肌细胞bcl2及bax相关凋亡因子的表达 7 1 材料与方法 8 2 结 果 9 3 讨 论 9 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 青蒿琥酯对佐剂性关节炎滑膜组织中凋亡因子FasFasL及Bcl 文1：青蒿琥酯对佐剂性关节炎滑膜组织中凋亡因子FasFasL及Bcl The Experimental Research on the Expression of Fas/FasL and Bcl2/Bax Correlated with Apoptosis in Synoviocyte of Adjuvant Arthritis with Artesunate Abstract：ObjectiveTo observe the expression of Fas/FasL and Bcl2/Bax correlated with apoptosis in synoviocyte of adjuvant arthritis (AA) with artesunate. MethodsSixty wistar rats were randomly divided into six groups:normal group, model control group, artesunate group I(high dose), artesunate group II(low dose), artesunate plus MTX group, MTX group. Each group was modeled to adjuvant arthritis with complete Freunds. After treated with artesunate or artesunate plus MTX, the arthrosis index(AI), the expression of Fas/FasL，Bcl2，Bax, which were correlated with apoptosis in arthrosis synovial membrane tissue were examined by immunohisto chemistry(IHC). ResultsCompared with the model control group, the AI in every experiment group decreased significantly(P). Compared with the model control group, the expression of Fas/FasL，Bax in arthrosis synovial membrane tissue increased significantly(P), Bcl2 decreased significantly(P). ConclusionThe effect of artesunate on the alleviation of adjuvant arthritis(AA) was proved in experiment, inducing apoptosis in synoviocyte was likely one of mechanisms. Key words：Artesunate; Rats; Arthritis; Apoptosis 青蒿琥酯（artesunate）是青蒿素的衍生物之一，为我国自主知识产权的高效抗疟药，有学者将其试用于 治疗 红斑狼疮等结缔组织疾病取得了较好的疗效［1］，为了探讨青蒿琥酯对类风湿关节炎的作用，作者作了青蒿琥酯对佐剂性关节炎的药理作用、以及对佐剂性关节炎滑膜组织中凋亡因子Fas/FasL及Bcl2/Bax表达影响的实验观察，报道如下。 1 材料与方法 动物与分组 雄性Wistar大鼠60只，平均体重：(±)g,由华中科技大学同济医学院动物实验中心提供。随机分为6组：空白组（8只），对照组（模型组）（8只），青蒿琥酯I组（10只），青蒿琥酯 II组（8只），青蒿琥酯加甲氨喋呤组（简称青加甲组，8只），甲氨喋呤组（8只） 药品、试剂及仪器 完全Freud佐剂,美国SIGMA公司 093K8932;青蒿琥酯注射液，桂林南药股份有限