黄芪多糖对大鼠局灶性脑缺血再灌注损伤的保护作用.docVIP

黄芪多糖对大鼠局灶性脑缺血再灌注损伤的保护作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：黄芪多糖对大鼠局灶性脑缺血再灌注损伤的保护作用 1 1 材料与方法 2 2 结果 5 3 讨论与分析 5 文2：黄芩苷对大鼠肝损伤的保护作用 6 1 材料与方法 7 2 结果 8 3 讨论 8 参考文摘引言： 9 原创性声明（模板） 11 文章致谢（模板） 11 正文 黄芪多糖对大鼠局灶性脑缺血再灌注损伤的保护作用 文1：黄芪多糖对大鼠局灶性脑缺血再灌注损伤的保护作用 Protective Effect of Astragalus polysaccharide on Focal Cerebral Ischemia-Reperfusion Injury in Rats 【Abstract】Objective:To study the protective effect of Astragalus polysaccharide on focal cerebral ischemia-reperfusion in jury in :The models of focal brain ischemia-reperfusion injury was prepared by middle cerebral artery occlusion(MCAO)in rats were randomly divided into sham operation control group,model group,low,middle and high dose drug group,after treatment the brain water content and the level of SOD,MDA were measured at the fit,third and seventh day after range of cerebral inflarct was observed by TTC :Compared with the sham operation control group,the brain water content and the level of MDA were significantly higher,and the level of SOD was significantly lower in model brain water content and the level of MDA were significantly lower,and the level of SOD was obviously higher in low,middle,high dose groups than ones in model :Protective effect of Astragalus polysaccharide on focal cerebral ischemia-reperfusion in jury in rats was remardable by reducing the brain water content and the level of MDA,and increasing the level of SOD. 【Key words】Astragalus polysaccharide;Cerebral Ischemia-Reperfusion Injury;SOD;MDA 黄芪多糖（Astragalus polysaccharide,APES）是从传统中药黄芪中提取的具有较强生物活性的大分子化合物，对造血系统、心血管系统、免疫系统、物质代谢系统以及保肝护肾、抗病毒、抗肿瘤、抗应激等多方面具有药 理学 活性[1]。其对缺血脑损伤的影响作用，已经引起人们的重视。本实验通过线栓法制备大鼠局灶性脑缺血（MCAO）再灌损伤模型，研究黄芪多糖对缺血脑损伤中脑组织含水量、SOD、MDA含量的影响。 1 材料与方法 材料 实验动物 SD大鼠，雌雄各半，体重250～300g，购自安徽长临河医药科技有限公司（普通级） 药品与试剂 APES由博泰生物科技有限公司提供，纯度95%；SOD试剂盒（批号：），MDA试剂盒（批号：），考马斯亮兰蛋白试剂盒（批号：）均购自南京建成生物工程研究所；水合氯醛（批号：）上海五联化工厂；TTC（Amresco 07/2010）；直径鱼线（日本PATU公司） 仪器 7200型紫外分光光度计（尤尼柯上海仪器有限公司）；冷冻离心机（3K30 sigma）；恒温培养箱（HPX-9272MBE 常州诺基仪