番茄红素对急性肺损伤大鼠肺泡巨噬细胞影响.docVIP

番茄红素对急性肺损伤大鼠肺泡巨噬细胞影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：番茄红素对急性肺损伤大鼠肺泡巨噬细胞影响 1 1 材料与方法 2 13 Wistar大鼠肺泡巨噬细胞的获取 3 131 支气管肺泡灌洗 参照 文献 〔7〕进行。 3 2 结果 4 3 讨论 4 文2：大承气汤对急性出血坏死性胰腺炎急性肺损伤大鼠肺泡巨噬细胞的影响 5 1 材料与方法 6 2 结果 9 3 讨论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 13 正文 番茄红素对急性肺损伤大鼠肺泡巨噬细胞影响 文1：番茄红素对急性肺损伤大鼠肺泡巨噬细胞影响 Effect of lycopene on function of pulmonary alveolar macrophages of rats acute lung injury Abstract： Objective To study the effect of lycopene on the function of pulmonary alveolar macrophages(PAMs)of rats acute lung injury(ALI).Methods Male rats were gavaged continuously by lycopene with 05,10,30mg/kg for 30 days;Lipopolysaccharide was used to make the model of ALI 1hour,4 and 6hou,bronchoalveolar lavage was done，and the function of PAMs,the amount of PAMs and the lung relative weight were Lycopene could increase the phagocytic function of PAMs significantly .Compared with the model group,the phagocytic function of PAMs was increased significantly in lycopene groups,with increasing rates of 112%,279%,381% after injection of LPS for 1 hour,with increasing rates of 59%,44%,170% after injection of LPS for 4 hou,and with increasing rates of 166%,152%,219% after injection of LPS for 6 hou,respectivelely compared with the model phagocytic function of PAMs was increased obviously by three different dose of lycopene,showing a doserespoe relatiohip(P005).Conclusion Lycopene,at the dose of 05,10 and 30mg/kg,could reinforce the phagocytic function of PAMs. Key words： lycopene;acute lung injury(ALI);pulmonary alveolar macrophages(PAMs)；phagocytic function 研究显示，番茄红素具有抗氧化、预防肿瘤和心血管疾病以及调节机体免疫功能等多种生理功能，是良好的抗氧化剂〔1-6〕。为观察番茄红素对脂多糖(lipopolysaccharide,LPS)致急性肺损伤(acute lung injury,ALI)大鼠肺泡巨噬细胞(pulmonary alveolar macrophages,PAMs)吞噬功能的影响，为番茄红素的开发提供基础数据进行了本研究。 1 材料与方法 11 主要仪器与试剂 LPS(美国Sigma公司)。番茄红素由北京联合大学生物活性与功能食品重点实验室应用超临界流体萃取技术分离纯化，纯度95%。RPMI1640培养基(美国Hy Clionepierce公司)。小牛血清(天津市财慧生化制品厂)。其他试剂均为国产分析纯。B80型低速自动平衡离心机(河北省安新县白洋离心机厂)；724微机型可见光分光光度计(上海光