何首乌蛋白质和蒽醌苷对D半乳糖衰老小鼠学习记忆及代谢产物的影响医学.docVIP

何首乌蛋白质和蒽醌苷对D半乳糖衰老小鼠学习记忆及代谢产物的影响医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：何首乌蛋白质和蒽醌苷对D半乳糖衰老小鼠学习记忆及代谢产物的影响医学 2 1 材料与方法 3 11动物 3 12药物与试剂 3 13仪器 4 14分组与给药 4 15观察指标与检测方法 5 151学习速率以及记忆能力测试 5 152相关酶及代谢产物检测 5 22各组对衰老小鼠学习速率及记忆能力的影响 6 23各组对衰老小鼠脏器系数的影响 6 文2：蜂胶总黄酮对D半乳糖诱导衰老小鼠学习记忆及代谢产物的影响临床医学 7 1 材料与仪器 8 2 方法 9 3 结果 10 4 讨论 11 参考文摘引言： 12 原创性声明（模板） 14 文章致谢（模板） 14 正文 何首乌蛋白质和蒽醌苷对D半乳糖衰老小鼠学习记忆及代谢产物的影响医学 文1：何首乌蛋白质和蒽醌苷对D半乳糖衰老小鼠学习记忆及代谢产物的影响医学 Abstract: ObjectiveTo observe the effects of protein and anthraquinones glycosides from Radix Polygoni Multiflori (RPM) on learning and memory as well as associated enzymes and metabolites in mice with Dgalactoseinduced aging. Methods Eighty Kunming mice were equally randomized into 8 groups: normal group, model group, vitamin E (90mg·kg-1·d-1) group, highand lowdose RPM protein (20 and5 mg·kg-1·d-1 respectively) groups, high and lowdose RPM anthraquinone glycoside (28 and 7mg·kg-1·d-1 respectively) groups, combination (lowdose RPM protein and lowdose RPM anthraquinone glycosides)group. Except the normal group,the mice in other groups received subcutaneous injection of Dgalactose to indue the aging learning rate of mice was detected with trisection radiation maze, memory was evaluated by onetime passive avoidance respoe, cerebral superoxide dismustase (SOD) and monoamine oxidase (MAO) activities as well as malondialdehyde（MDA）content were detected with ultraviolet spectrophotometer, and hepatic lipofuscin (LF) content was examined with fluorescence spectrophotometer. Results Memory of aged mice was much improved in highdose RPM protein group (P＜005), and learning rate and memory were improved in highdose RPM anthraquinone glycosides group (P＜005 or P＜001). RPM protein and anthraquinone glycosides increased liver index, thymus index, brain index and spleen index of aged mice (P＜005 or P＜001), and decreased cerebral MAO activity, MDA content and hepatic LF content