探讨改良线栓对局灶脑缺血模型制备的影响.docVIP

探讨改良线栓对局灶脑缺血模型制备的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：探讨改良线栓对局灶脑缺血模型制备的影响 1 0 引言 2 1 材料和方法 3 2 结果 5 3 讨论 6 文2：胎儿微嵌合体对Graves病动物模型制备的影响 8 1 材料与方法 9 2 结 果 11 3 讨 论 13 参考文摘引言： 14 原创性声明（模板） 16 文章致谢（模板） 16 正文 探讨改良线栓对局灶脑缺血模型制备的影响 文1：探讨改良线栓对局灶脑缺血模型制备的影响 【Abstract】 AIM: To investigate the application of modified suture in preparation of rat model of focal cerebral ischemia and to evaluate the stability and reliability of the model. METHODS: 40 Wistar male rats were randomized into the conventional suture group and the modified suture group, with 20 in each group. The focal cerebral ischemia by MCAO was induced according to Longas suture method. The effects of the application of modified suture on the total operation time, the common carotid artery blood occlusion time during the coue of operation, the probability that suture entered the pterygopalatine artery (PPA) and the success rate were observed. And the stability and the reliability of the model were evaluated by neurological deficit score, magnetic resonance image, triphenyltetrazolium chloride (TTC) staining in all rats. RESULTS: The total operation time and the common carotid artery blood occlusion time in the modified suture group were obviously shorter than those in the conventional suture group. And the application of modified suture in the model cut down the probability that the suture entered PPA, and increased the success rate, the stability and the reliability of the model. CONCLUSION： The application of modified suture could improve the success rate, the stability and the reliability of the rat focal cerebral ischemia induced by MCAO. 【Keywords】 brain ischemia; disease models, animal; suture techniques; middle cerebral artery occlusion; rats 0 引言 大鼠的基因与人类的基因具有98%的同源性，其脑血管结构与人类非常相似，血管闭塞后缺血范围恒定、重复性好. 因此，众多有关脑缺血的研究均选用大鼠来制备模型［1-2］. 制备大鼠局灶脑缺血模型的方法较多，但以线拴法应用最广. 线栓法制备局灶脑缺血模型目前还存在某些不足，如线栓插入困难，栓塞成功率不高，梗塞体积不稳定等［3］. 这与线栓直径、弧度以及术中颈总动脉血流阻断时间等因素密切相关.本研究重在对线栓弧度进行改良，观察改良线栓对局灶脑缺血模型的