血管性痴呆大鼠脑内超微结构变化及生长抑素和nNOS的表达临床医学.docVIP

血管性痴呆大鼠脑内超微结构变化及生长抑素和nNOS的表达临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：血管性痴呆大鼠脑内超微结构变化及生长抑素和nNOS的表达临床医学 1 1 材料与方法 3 2 结 果 4 3 讨 论 5 文2：血管性痴呆大鼠海马碱性成纤维细胞生长因子的表达临床医学 7 1 材料与方法 8 2 结果 9 3 讨论 9 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 血管性痴呆大鼠脑内超微结构变化及生长抑素和nNOS的表达临床医学 文1：血管性痴呆大鼠脑内超微结构变化及生长抑素和nNOS的表达临床医学 【Abstract】 Objective To study the changes of the somatostatin (SS), nitric oxide synthase (nNOS) of thevascular dementia (VD) rats, and to explore the relatiohip between them and the cognitive function. Methods 30 14～15monthold rats were randomly divided into sham operation (SOG) and model groups (MDG), MDG group was ligated bilateral carotid artery by thread to prepare for the animal model of the chronic front cerebral ischemia. SOG group was only separated bilateral main carotid artery, but was not blocked the blood flow. Water maze test was used to test the abilities of spatial learning and memory of the rats. The tramission electron microscope was used to observe ultrastructural changes in rat neuro; immunohistochemical method was adopted to detect the SS and nNOS expressio in hippocampus and temporal cortex. Results In the water maze test, the escape latency of the MDG rats 〔(±)s〕 was significantly longer than that of SOG group 〔(±)s, P〕，the number of crossplatform of MDG rats (±) was decreased significantly compared with that in SOG group 〔(±), P〕. TEM observation showed that there was high degree of edema in hippocampal neuro, karyopyknosis or collapse, and mitochondrial swelling. Immunohistochemistry results showed that the expression of SS in hippocampus was decreased significantly in MDG group(±)compared with that in SOG group 〔(±), P〕, the expression of SS in temporal lobe was decreased significantly in MDG group(±)compared with that in SOG group 〔(±), P〕 , the expression of nNOS in hippocampus was increased significantly in MDG group(±)compared with that in SOG group 〔(±), P〕, the expression of nNOS in temporal lobe was incr