辛伐他汀对糖尿病大鼠MMP9表达的影响及对肾脏保护的研究临床医学.docVIP

辛伐他汀对糖尿病大鼠MMP9表达的影响及对肾脏保护的研究临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：辛伐他汀对糖尿病大鼠MMP9表达的影响及对肾脏保护的研究临床医学 1 1 材料与方法 3 2 结果 5 3 讨论 7 文2：辛伐他汀对糖尿病大鼠肾脏活化蛋白1表达的影响临床医学 8 1 材料与方法 9 2 结果 12 24 h尿蛋白、Cr、Glu、TC、TG 12 3 讨论 13 参考文摘引言： 14 原创性声明（模板） 16 文章致谢（模板） 16 正文 辛伐他汀对糖尿病大鼠MMP9表达的影响及对肾脏保护的研究临床医学 文1：辛伐他汀对糖尿病大鼠MMP9表达的影响及对肾脏保护的研究临床医学 【Abstract】Objective To observe the effects of simvastatin on the expression of matrix metalloproteinase9 (MMP9) mRNA in renal tissue and urinary MMP9 excretion of diabetic rats and find its renal protective mechanisms. Methods 24 Wistar rats were randomly divided into control, diabetic, simvastatin treament groups. Peripheral blood glucose, HbAlc, the urinary albumin (ALB), retinalbinding protein (RBP) and MMP9 were tested at 8th week. The renal tissue of rats were obtained for renal pathological changes by electron microscope and RTPCR to examine the expression of MMP ①At the 8th week, the urine excretion rates of ALB, RBP and MMP9 in diabetic and simvastatin treament groups were higher than those in control group (P＜). In simvastatin treament group, they were decreased significantly than those in diabetic group (P＜). Urinary MMP9 level was positive correlated with the urinary ALB and RBP levels. ②The expression of MMP9 mRNA was markedly upregulated in diabetic group compared with that in control group, which in simvastatin treament group was much lower than that in diabetic group (P＜)，the renal pathological changes in simvastatin treament group were much lighter than those of diabetic group, but a little heavier than those of control group. Conclusio Simvastatin has a renalprotection effect, which may be associated partly with downregulated overexpression of renal tissue MMP9. 【Key words】Simvastatin; Diabetes mellitus; Diabetic nephropathy; Matrix metalloproteinase9 糖尿病肾病(diabetic nephropathy，DN)是糖尿病常见的微血管并发症，是导致糖尿病患者致死致残的主要原因之一。DN的发病机制尚不十分明确，包括有代谢紊乱，肾小球血流动力学异常和遗传易感性等方面。糖尿病肾组织细胞外基质成分堆积与肾内高糖环境