亚低温治疗对大鼠缺血性脑损伤后热休克蛋白表达的影响.docVIP

亚低温治疗对大鼠缺血性脑损伤后热休克蛋白表达的影响 文档信息 属性： Doc-03D00F，doc格式，正文11192字。质优实惠，欢迎下载！ 说明： 作为医药学资料、临床医学资料的写作参考资料，提供解决怎么写及格式等相关问题。 适用： 作为文章写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：亚低温治疗对大鼠缺血性脑损伤后热休克蛋白表达的影响 2 1 材料与方法 3 2 结果 5 3 讨论 6 文2：亚低温治疗对大鼠缺血性脑损伤后热休克蛋白表达的影响 8 1 材料与方法 9 2 结果 11 3 讨论 12 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 15 正文 亚低温治疗对大鼠缺血性脑损伤后热休克蛋白表达的影响 文1：亚低温治疗对大鼠缺血性脑损伤后热休克蛋白表达的影响 Abstract Objective: To investigate the effectiveness of mild hypothermia therapy on heat shock protein(Hsp) expression in rats with focal cerebral ischemia. Methods: 48 SD rats were randomly pided into the trial group(the mild hypothermia group) and the control group. Each group was subpided into 3 groups (sub-groups) according to the post-infarction disposal time， with 8 SD rats in each sub-group. The rat models of focal cerebral ischemia were established with the modified Longa method. The rats in the trial group were cooled by mild hypothermia for two hou， while those in the control group were subjected to no disposal. The rats in every sub-group was killed at the points of 12 hou， 24 hou， 48 hou after infarction， respectively. Immunohistochemistry method was performed to confirm the expression of the Hsp 70 at thepenumbra fieldin rat brain tissue. Results: The expression of the Hsp70 in rats of the trial group was less than that of the control groups at each time point ， there was significant difference in the expression of Hsp70 between the 24 hou subgroup and the 48 hou subgroup (P Key words Mild hypothermia; brain ischemia; Heat shock protein 70 低温一般分为轻度低温(mild hypothermia)(33～35℃)；中度低温(moderate hypothermia)(28～32℃);深度低温(profound hypothermia)(17～27℃);超深低温(ultraprofound hypothermia)(2～16℃)。亚低温一般指32～35℃的低温，亚低温脑保护的研究已有数十年历史，脑组织热休克蛋白70 (Heat-shock protein 70，Hsp70)主要存在于缺血半暗带，本研究通过观察亚低温处理后实验大鼠缺血区脑组织内Hsp70含量的变化，探讨亚低温的脑保护机制。 1 材料与方法 动物分组 健康SD大鼠雌雄不计共48只，体重300～330g，随机分为两组：亚低温组和常温组，每组在术后又分为12小时、24小时、48小时3个亚组。 脑缺血模型的制备 参照改良的Lon