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REV. A 1 OF 3
Replacing the 2475 Flow Cell
When to Replace
• If the flow cell is damaged
Parts Required
• Flow Cell Assembly, # 700001618
Tools Required
• Flat-blade screwdriver, WAT272058
Preparation
1. Before performing this procedure, you should flush and dry the flow cell by following
the “Flushing the Flow Cell” procedure on this CD, or refer to the 2475 Multiwave-
length Fluorescence Detector Operators Guide.
2. Always wear powder-free gloves or finger cots when handling the flow cell to prevent
contact with residual solvents. W
Removing the Flow Cell Assembly
1. Turn the detector off, then disconnect the power cord.
E
2. Remove the left front cover. I
3. Remove the tubing from the strain relief.
4. Using the flat-blade screwdriver, loosen the three captive screws on the flow cell
V
assembly front plate. (Figure 1).
E
R
Figure 1 - Captive Screw Location
Replacing the 2475 Flow Cell
REV. A 2 OF 3
5. Gently pull the flow cell assembly straight out to remove it.
Installing the Flow Cell Assembly
1. Insert the new flow cell into the detector.
2. Alternately finger-tighten the captive screws, to ensure that the flow cell seats
properly. Then tighten with the flat-head screwdriver.
3. Put the tubing back into the strain relief, and replace the left front cover.
4. Replace the left-front detector panel.
5. Reconnect the power cord and power on the detector.
6. Allow the detector to stabilize for at least one hour.
Ensuring Detector Operation
Before performing any chromatography, verify detect
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