巴拿巴对糖尿病模型小鼠血糖的影响.docVIP

·637 · · 论 著 · 巴拿巴对糖尿病模型小鼠血糖的影响 方洁，张爱珍，陈建国 【摘要】 目的 探讨巴拿巴软胶囊对糖尿病小鼠血糖的影响。方法 选取200只ICR 小鼠，随机抽取30只小鼠 作为正常对照组 (NC 组),其余小鼠以200mg/kg 四氧嘧啶腹腔注射造成糖尿病模型，72 h 后取血糖在15～30 mmol/L 的小鼠120只，随机分成糖尿病模型组 (MC 组)、巴拿巴高剂量组 (HD 组)、巴拿巴中剂量组 (MD 组)、巴拿巴低 剂量组 (LD 组),各30只； NC 组小鼠腹腔注射等体积0.9%氯化钠注射液。然后巴拿巴按100、20、4 mg ·kg?1 · d? 溶于5 ml 色拉油中分别对HD 组 、MD 组、 LD 组小鼠灌胃， MC 组和NC 组小鼠给予等剂量色拉油灌胃。连续进行 42d, 观察不同时间巴拿巴软胶囊对小鼠空腹血糖、胰岛素的影响。结果 各组小鼠给药前及给药后不同时间空腹血 糖水平比较，差异有统计学意义 (F 时间xa=25.166,P0.01;Fm=10.318,P0.01;Fmm=11.250,P0.01); 给药后第14天， LD 组、 HD 组小鼠空腹血糖低于MC 组，给药后第28天LD 组、MD 组、 HD 组小鼠空腹血糖均低于 MC 组，给药后第42天MD 组、 HD 组小鼠空腹血糖低于MC 组，组间比较差异均有统计学意义 (P0.05)。 给药后第 42天各组小鼠胰岛素水平比较，差异有统计学意义 (F=4.232,P=0.005); 其 中HD 组小鼠高于MC 组，差异有统 计学意义 (P0.05)。 结论巴拿巴软胶囊能明显降低四氧嘧啶糖尿病小鼠空腹血糖水平，且有剂量、时间依赖性； 另外，还能促进胰岛素分泌。 【关键词】 巴拿巴；糖尿病；四氧嘧啶；小鼠 【中图分类号】 R587.1 【文献标识码】 A doi:10.3969/j. issn.1007-9572.2013.02.090 Effect of Banaba Capsules on Diabetes Mellitus of Model Mice FANG Jie,ZHANG Ai-zhen,CHEN Jian -guo. Zhejiang University City College School of Medicine,Hangzhou 310015, China 【Abstract 】 Objective To explore the effect of Banaba capsules on the blood glucose of alloxan induced diabetic mice. Methods A total of 200 ICR mice were involved. 30 mice were selected as NC group and other mice were established as di- abetes models by intraperitoneal injection of 200 mg/kg alloxan. After 72 h, 120 mice with blood glueose from 15 to 30 mmol/L were randomly divided into MC group, HD group, MD group and LD group with each group 30 mice. The NC group was given 200 mg/kg sodium chloride injection. The HD group, MD group and LD group were given intragastric administration of 100,20 and 4 mg ·kg- 1 ·d-Banaba capsules dissolved in 5 ml salad oil respectively. The MC group and NC group were given intragas- tric administration of the same volume of salad oil. The treatment was given for 42 consecutive days and the effect of Banaba cap- sule on fasting blood glucose and insulin at different times was observed. Results The fasting blood glucose at