抵当芪桂汤对链脲佐菌素诱导糖尿病大鼠心肌超微结构的影响.docVIP

·1201 · · 论 著 · 抵当芪桂汤对链脲佐菌素诱导糖尿病大鼠心肌超微结构的影响 杨景锋，赵天才，任艳芸，李鹏，李月红 【摘要】 目的 观察链脲佐菌素 (STZ) 诱导糖尿病大鼠心肌组织超微结构的变化及抵当芪桂汤对其超微结构 的影响。 方法 健康雌性SD 大鼠50只，随机抽取10只为空白组，其余40只大鼠以STZ诱导成糖尿病模型。造模成 功后随机分为模型组、达美康组、抵当芪桂汤组，给予相应干预。8周后检测大鼠的血糖、血脂等生化指标，用H- 600透射电镜和普通光镜观察STZ大鼠心肌组织结构的变化。 结果 (1)造模成功后，模型组大鼠血糖明显升高 (P 0.01)。(2)干预结束后，与模型组比较，达美康组、抵当芪桂汤组大鼠心肌细胞肥大及坏死有所改善，细胞间质 减少，肌丝排列相对整齐，抵当芪桂汤组改善较明显。结论 糖尿病大鼠心肌超微结构损伤明显，抵当芪桂汤可以改 善STZ 诱导糖尿病大鼠心肌超微结构，从而延缓糖尿病心肌病变的发生和发展。 【关键词】 糖尿病大鼠；链脲佐菌素；心肌超微结构；抵当芪桂汤 【中图分类号】 R587.23 【文献标识码】 A 【文章编号】1007-9572(2010)04-1201-03 Effects of Didangqigui Recipe on Myocardial UItrastructure in Diabetic Rats Induced by Streptozotocin YAN G Jing - feng, ZHAO Tian-cai,REN Yan-yun, et al. Shanxi University of Chinese Medicine,Xianyang 712046,China 【Abstract 】 Objective To investigate the change of myocardial ultrastructure in streptozotocin(STZ) -induced dia- betic rats, and the protective effects of Didangqigui Recipe on myocardial ultrastructure. Methods Ten of 50 healthy male SD rats were chosen randomly as blank group, the other 40 were STZ -induced into diabetic models, which were randomized into groups model, gliclazide, Didangqigui after establishment, receiving corresponding intervention. The blood glucose, lipid and other biochemical indicators were detected, and the changes of myocardial tissue structure of STZ rats were observed by H-600 transmission electron microscope and ordinary light microscope. Results STZ rats had higher blood glucose after model estab- lishment(P0.01). The cardiac hyperrophy and necrosis improved, interstitial cells reduced,myofilament arrangement was relatively neat in groups gliclazide and Didangqigui as compared with model group after intervention, but Didangqigui group im- proved more. Conclusion Myocardial ultrastructure injury is obvious in diabetic rats. Didangqigui recipe can improve the myo- cardial ultrastructure of STZ-induced diabetic rats, thus to delay the occurrence and development of diabet