肝X受体表达对糖尿病肾病大鼠的影响及机制研究.docVIP

·1029 · · 论 著 · 肝 X 受体表达对糖尿病肾病大鼠的影响及机制研究 师帅帅，张毅强，温文斌，张巍，赵银娥 【摘要】 目的 探讨肝X 受体 (LXR) 对糖尿病肾病大鼠的影响及机制。方法 选取60只Wistar 大鼠，采用 抽签法随机分为对照组、模型组及干预组，每组各20只。模型组及干预组大鼠通过链脲佐菌素(60mg/kg) 腹腔注射 建立糖尿病肾病模型，干预组大鼠给予LXR 激动剂T0901317(30mg ·kg?1 ·d?1)。2 周后，称重，留取24 h 尿，之 后腹腔注射乙醚，在腹上动脉部位进行取血，随后处死，留存血液样本，测量血糖、肾功能、血脂水平。 结果 1 2 周 后，3组大鼠体质量、尿蛋白、血糖、肌酐、尿素氮、内生肌酐清除率、三酰甘油、总胆固醇、高密度脂蛋白胆固醇 水平比较，差异均有统计学意义 (P0.05); 其中模型组和于预组尿蛋白、血糖、肌酐、尿素氮、三酰甘油、总胆固 醇水平高于对照组，体质量、内生肌酐清除率、高密度脂蛋白胆固醇水平低于对照组 (P0.05); 干预组尿蛋白、血 糖、肌酐、尿素氮、三酰甘油、总胆固醇水平低于模型组，体质量、内生肌酐清除率、高密度脂蛋白胆固醇水平高于 模型组 (P0.05)。 结论 LXR 表达能够明显改善 Wistar大鼠糖尿病肾病生化指标，具有一定的指导意义。 【关键词】 糖尿病肾病；肝X 受体；血糖；肾功能试验；脂蛋白类 【中图分类号】 R587.24 R -332 【文献标识码】 A doi:10.3969/j.issn.1007-9572.2015.09.011 师帅帅，张毅强，温文斌，等.肝X 受体表达对糖尿病肾病大鼠的影响及机制研究 [J]. 中国全科医学，2015, 18(9):1029- 1031,1036.[] Shi SS, Zhang YQ, Wen WB, et al.Effects of liver X receptor expression on rats with diabetic nephropathy and its mechanism [J].Chinese General Practice,2015,18(9):1029- 1031,1036. Effects of Liver X Receptor Expression on Rats with Diabetic Nephropathy and Its Mechanism SHI Shuai - shuai, ZHANG Yi-qiang,WEN Wen -bin, et al. Department of Nephrology, Heji Haspital Affiliated to Changzhi Medical College, Changzhi 046011,China 【Abstract 】 Objective To investigate the effects of liver X receptor(LXR)expression on rats with diabetic nephropathy(DN)and its mechanism. Methods 60 Wistar rats were randomly divided into control group,model group and intervention group by sortition randomization method with 20 rats in each group. Rats in model group and intervention group were injected intraperitoneally with streptozocin(STZ)(60 mg/kg)injection to establish DN model. Rats in intervention group were treated with LXR agonists TO901317 (30 mg ·kg-.d-). After 12 weeks,the rats were weighed up, 24 - hour urine of rats was collected, then rats were injected intraperitoneally with ether, blood samples were taken from epigastric vessels, then rats were put to death, the