银杏提取物中萜类化合物黄酮类血管扩张作用比较comparative vasodilating actions among terpenoids and.pdfVIP

ClinicaChimicaActa339(2004)129133

Comparativevasodilatingactionsamongterpenoidsand

flavonoidscontainedinGinkgobilobaextract

SeiichiroNishida,HiroyasuSatoh*

DepartmentofPharmacology,DivisionofCrudeandHerbalMedicine,NaraMedicalUniversity,Kashihara,Nara634-8521,Japan

Received6August2003;receivedinrevisedform1October2003;accepted3October2003

Background:ComparativevasodilatingactionsoftheconstituentsofGinkgobilobaextract(GBE),terpenoids(bilobalide,

ginkgolidesA,BandC)andflavonoids(quercetinandrutin),wereexaminedusingrataortaringstrips.Methods:Cumulative

administrationsofGBEanditsconstituentswerefollowedwiththepretreatmentof5Amol/lNE.Results:GBEat0.03to3mg/

mlhadapotentconcentration-dependentrelaxation;by70F4.5%(n=6,P0.001)at3mg/ml.Terpenoidsandflavonoidsat

0.1to100Amol/lhadpotentconcentration-dependentrelaxation.At100Amol/l,bilobalidedilatedby17.6F3.9%(n=7,

P0.05),andginkgolidesA,BandCalsocausedittothealmostsameextent.Quercetin(100Amol/l)causedapotent

vasorelaxationby49.9F4.8%(n=10,P0.001).Rutinat100Amol/lhadweakervasorelaxation;by13.7F3.2%(n=6,

P0.01).Conclusions:AllconstituentsofGBEhavetheconcentration-dependentvasorelaxtanteffect.ThepotencyofGBE’s

actionwasnotmadesimplybyadditionofthoseoftheconstituents.EachconstituentitselfwouldcontributetotheGBE-

inducedvasodilation,althoughtheconstituentshavethecomplicatedinteractionswitheachother.

D2003ElsevierB.V.

Keywords:Terpenoids;Bilobalide;Flavonoids;Quercetin;Ginkgobilobaextract;Vasodilation

1.Introduction