HPLC和HPLC-MS_MS法测定白酒中甜蜜素的方法建立与比较.pdfVIP

AnalysisandTesting分析检测

doi:10.16736/41-1434/ts.2025.11.041

HPLC和HPLC-MS/MS法测定白酒中

甜蜜素的方法建立与比较

EstablishmentandComparisonofMethodsforDeterminingCyclamateinBaijiubyHPLCandHPLC-

MS/MS

◎沈蕊111111122

，胡杨，毛琼丽，黄智安，熊晓通，解倩倩，罗欣，杨强，李强

（1.劲牌有限公司，湖北 黄石 435100；

2.中药保健食品质量与安全湖北省重点实验室，湖北 黄石 435100）

SHENRui111111

,HUYang,MAOQiongli,HUANGZhian,XIONGXiaotong,XIEQianqian,

122

LUOXin,YANGQiang,LIQiang

(1.JingBrandCo.,Ltd.,Huangshi435100,China;

2.HubeiKeyLaboratoryofQualityandSafetyofTraditionalChineseMedicineHealthFood,Huangshi435100,China)

摘要：High-PerformanceLiquidChromatographyHPLC-

High-PerformanceLiquidChromatography-TandemMassSpectrometryHPLC-MS/MS

92.2%109.4%

0.12%2.80%HPLC0.006g·kg-1HPLC-MS/MS0.0495mg·kg-1

HPLCHPLC-MS/MSHPLC-MS/MS

HPLC

关键词：-

Abstract：Objective:Toestablishhighperformanceliquidchromatography(HPLC)andhighperformanceliquid

chromatographytandemmassspectrometry(HPLC-MS/MS)forthedeterminationofsodiumcyclamateinBaijiu.Method:

Finetunethepre-processingsteps,chromatographicconditions,andmassspectrometryparameterconfiguration,and

systematicallyinvestigatetheperformanceindicatorssuchasdetectionlimit,quantificationlimit,repeatability,stability,and

accuracyofthetwomethods.Result:Therecoveryratesofthetwomethodsrangedfrom92.2%to109.4%,witharelative

standarddeviationof0.12%to