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Resazurinsodium(solution)

Cat.No.:HY-DY1051

CASNo.:62758-13-8

分⼦式:C₁₂H₆NNaO₄

分⼦量:251.17

作⽤靶点:Bacterial;FluorescentDye

作⽤通路:Anti-infection;Others

储存⽅式:Pleasestoretheproductundertherecommendedconditionsin

theCertificateofAnalysis.

BIOLOGICALACTIVITY

⽣物活性Resazurinsodium(Diazoresorcinolsodium)(solution)是⼀种⽆毒的、稳定的膜渗透性蓝⾊⾮荧光染料(有微

弱的荧光)。Resazurinsodium作为氧化还原指⽰剂，在活细胞中能被还原成粉⾊的具有⾼度荧光的Resorufin

sodium(Ex=530-560nm,Em=590nm)，从⽽⽤于细胞(⼈、动植物、细菌和真菌)活⼒、毒性、增殖、迁移

和侵袭的检测。

溶剂及浓度：⽆菌⽔：10mM

体外研究Resazurinsodium(Diazoresorcinolsodium)iscommonlyusedtomeasurebacterialandeukaryoticcell

viabilitythroughitsreductiontothefluorescentproductresorufin.Noviablebacteriaaredetected24hpost-

inoculationfollowinginclusionofResazurinsodiuminTSBcculturesofF.tularensisLVSattheconcentration

of44μM.LoweringtheResazurinsodiumconcentrationtoaslittleas4.4μMstillresultsina10-fold

reductioninviableF.tularensisLVScomparetogrowthmediumalone.BothResazurinsodiumtreatments

resultinasignificantdecreaseinviableF.tularensisLVSbacteriaover22h.Tr