内质网应激相关基因mRNA在2型糖尿病合并脂肪肝小鼠肝脏中的表达变化.docVIP

  内质网应激相关基因 mRNA 在 2 型糖尿病 合并脂肪肝小鼠肝脏中的表达变化# 成细华1，喻嵘2** 5 （1. 湖南中医药大学医学院,长沙,410208； 2. 湖南中医药大学中医学院,长沙,410208） 摘要：目的 探讨内质网应激在高脂饮食 2 型糖尿病转基因 MKR 小鼠脂肪肝发生中的作用。 方法 高脂饲料诱发 MKR 鼠形成非酒精性脂肪肝模型，观察肝脏形态变化，检测肝脏甘油 三酯和脂肪酸水平，RT-PCR 检测小鼠肝脏内质网应激相关基因 mRNA 表达变化。结果 与 10 正常小鼠比较，MKR 组小鼠肝脏 GRP78、XBP1、FAS、ACC-α和 GSK3β mRNA 表达水 平有升高趋势（P0.05）；与 MKR 小鼠比较，高脂饮食 MKR 小鼠肝脏甘油三脂和脂肪酸 水平显著升高，肝脏 GRP78、XBP1、CHOP、SREBP1c 、FAS、ACC-α、GSK3β和 EDEM1 基因 mRNA 表达水平均增加（P0.01），而 apoB100 mRNA 表达水平降低（P0.01）。结 论 ERS 参与调节肝脏脂质及 apoB100 的代谢过程，在转基因 2 型糖尿病 MKR 鼠脂肪肝形 15 20 25 30 35 成中发挥重要作用。 关键词： 内质网应激； 糖尿病合并脂肪肝； MKR 小鼠； 脂代谢 中图分类号：请查阅《中国图书馆分类法》 The related gene expressions of endoplasmic reticulum stress in the liver of type II diabetes mice combined with liver steatosis Cheng Xihua1, YuRong2 (1. Hunan University of Traditional Chinese Medicine , Changsha , 410208; 2. Hunan University of Traditional Chinese Medicine , School of Medicine , Changsha , 410208) Abstract: Objective To observe the effect of endoplasmic reticulum stress on the formation of liver steatosis in the type 2 diabetes MKR mice raised by high fat feeds. Method MKR mice were raised by high fat feeds to form liver steatosis. The pathological changes of the liver were examined, and the levels of TG and FFA in the liver were examined too. RT-PCR was used to analyze the related gene expressions of endoplasmic reticulum stress in the liver. Results The gene expressions of GRP78, XBP1, FAS, ACC-α and GSK3β on mRNA level in MKR mice were elevated slightly in contrast with normal mice（P0.05）;The levels of TG and FFA in the liver were increased remarkably, and the gene expressions of GRP78, XBP1, CHOP, SREBP1c, FAS, ACC-α, GSK3β and EDEM1 on mRNA level in MKR mice with liver steatosis were elevated significantly in contrast with MKR mice （P0.01）, while the gene expression of apoB100 was decreased（P0.01）. Conclusion There has a close relationship between the liver steatosis and the involvment of ERS in the lipid metabolism in the liver of MKR mice. Key wo