兔隐孢子虫生物学特性研究.docVIP

  兔隐孢子虫生物学特性研究# 齐萌，吴国泉，菅复春，李俊强，王荣军，宁长申，张龙现** （河南农业大学牧医工程学院，郑州 450002） 5 10 摘要：目的 为研究新近发现的人兽共患隐孢子虫种兔隐孢子虫的生物学特性。方法 基于显 微镜观察，自然宿主和试验动物宿主特异性，病理组织学观察等方面进行研究。结果 兔隐 孢子虫可成功感染日本大耳白兔和免疫抑制蒙古沙鼠，而未免疫抑制蒙古沙鼠、免疫抑制 BABL/c 小鼠、免疫正常 BABL/c 小鼠和雏鸡均未发现感染。不同剂量卵囊接种 30 日龄兔后， 潜隐期为 4-5d，显露期 6-11d，各感染组兔均未出现腹泻症状；90 日龄兔和免疫抑制组蒙古 沙鼠接种 2.0×105 个卵囊后，潜隐期均为 5d，显露期分别为 7d 和 5d，未表现临床症状。 组织学观察可见，兔隐孢子虫在兔和蒙古沙鼠体内的寄生部位均为空肠和回肠，虫体寄生部 位肠绒毛脱落或变短。结论 兔隐孢子虫可感染自然宿主和免疫抑制蒙古沙鼠，成功建立兔 隐孢子虫的兔感染模型。 关键词：预防兽医学；兔隐孢子虫；生物学特性 15 Study on the biological Characterization of Cryptosporidium Cuniculus Qi Meng, Wu Guoquan, Jian Fuchun, Li Junqiang, Wang Rongjun, Ning Changshen, Zhang Longxian 20 25 30 35 40 (College of Animal Science and Veterinary Medicine, Henan Agricultural University, ZhengZhou 450002) Abstract: In this paper, the objective is to study the biological characterization of the newly emerging zoonotic Cryptosporidium cuniculus. Methods The morphology, natural and experimental host specificity, and histological examination were investigated. Results Experimental infections have been established in Japanese White Rabbit and immunosuppressed Mongolian gerbil, but not in normal immune Mongolian gerbil, immunosuppressed BABL/c mice, normal immune BABL/c mice and chickens. Different dosage of C. cuniculus oocysts were respectively inoculated to 30-day-old rabbits, and the latent period and the patent period was 4-5 days and 6-11 days, respectively. No diarrhea was noticed in each rabbit. 2×105 oocysts of C. cuniculus were infected to 90-day-old rabbits and immunosuppressed Mongolian gerbil, the latent period was all 5 days, and the patent period was 7 days and 5 days, respectively, with the absence of clinical signs. Histological examination showed the parasites were located in the jejunum and ileum, with the intestinal villi being atrophied or desquamated. Conclusion Cryptosporidium cuniculus could infect natural host and immunosuppressed Mongolian gerbil, and the infection model using rabbit was successfully esta