枸杞叶总黄酮对人肝癌细胞HepG2增殖与凋亡的影响.docVIP

  枸杞叶总黄酮对人肝癌细胞 HepG2 增殖与 凋亡的影响# 雷蕾1，袁国强2，刘健锋1，王长江1，岳明霞1，秦崧1，张璐1，王进宝1，邵宝平 5 1 ，王建林1** （1. 兰州大学生命科学学院，兰州 730000； 2. 兰州大学第二医院神经病学研究所，兰州 730030） 摘要：【目的】研究枸杞叶总黄酮对体外培养的人肝癌细胞 HepG2 增殖抑制及其细胞凋亡的 10 15 20 25 30 35 40 45 影响。【方法】使用不同浓度的枸杞叶总黄酮作用于体外培养的人肝癌细胞 HepG2，用四甲 基偶氮噻唑蓝(MTT)法检测 HepG2 细胞存活率, 通过 Hoechst33258 染色法，AV/PI 双染流式 细胞术检测 HepG2 细胞凋亡情况,利用免疫细胞化学法分析枸杞叶总黄酮对 HepG2 细胞凋亡 相关蛋白 Bax、caspase-3 表达的影响。【结果】枸杞叶总黄酮分别作用于人肝癌细胞 HepG2 48h、72h 均可明显抑制其增殖，并呈现显著的时间、剂量依赖性。高浓度枸杞叶总黄酮作 用人肝癌细胞 HepG2 48h 后，HepG2 细胞呈现出典型的细胞凋亡特征，凋亡率可高达 67.3%。 枸杞叶总黄酮作用于 HepG2 细胞一定时间后，可引起促凋亡蛋白 Bax、caspase-3 的表达上 调。【结论】枸杞叶总黄酮可明显抑制人肝癌细胞 HepG2 增殖和诱导细胞凋亡,其作用机制 可能是通过有效上调促凋亡蛋白 Bax，破坏细胞内稳态环境，继而激活凋亡蛋白 caspase-3， 最终引发人肝癌细胞 HepG2 凋亡。 关键词：人肝癌细胞 HepG2；枸杞叶总黄酮；凋亡；Bax；caspase-3 中图分类号：Q95 Effects of total flavonoids in Lycium chinensis leaves on proliferation and apoptosis of hepatoma cell line HepG2 in vitro LEI Lei1, YUAN Guoqiang2, LIU Jianfeng1, WANG Changjiang1, YUE Mingxia1, QIN Song1, ZHANG Lu1, WANG Jinbao1, SHAO Baoping1, WANG Jianlin1 (1. School of Life Science, Lanzhou University, LanZhou 730000; 2. Institute of neurology of the second hospital, LanZhou university, LanZhou 730030) Abstract: [Aims]The goal of this study was to investigate the effects of total flavonoids in Lycium chinensis leaves on cell proliferation and apoptosis of human hepatocellular carcinoma (HepG2 cells) cultured in vitro. [Methods] HepG2 cells were treated with different doses of total flavonoids in Lycium chinensis leaves, the cell viability was measured using MTT assay. The morphological changes of the apoptotic cells were assessed by fluorescent Hoechst 33258 stain. The apoptotic rate was measured by flow cytometry. The apoptosis-associated protein expression levels of Bax and Caspase-3 were analyzed by immunocytochemistry method. [Results] The total flavonoids in Lycium chinensis leave can dose-dependently and time–dependently inhibit the cell viability of HepG2 effectively when incubated either for 48h or for 72h. The HepG2 was treated w