武运粳7号超表达OsNRT1.2后对硝酸盐的响应.pdfVIP

中国水稻科学 (ChinJRiceSci)，201】，25(4)：349～356 http1}|www．ricesci．cn DOI：10．3969／j．issn．1001—7216．2011．04．002 349 武运粳 7号超表达 OsNRT1．2后对硝酸盐的响应 马 翠 范晓荣 徐国华 (南京农业大学 资源 与环境科学学 院，江苏 南京 210095； 通讯联系人 ，E—mail：xiaorongfan@njau．edu．cn) ResponsesofRicePlantsofWuyunjing7toNitrateasAffectedbyOver—Expressionof oSNRT1．2 MACui，FANXiaorong ，XuGuo—hua (CollegeofResourcesandEnvironmentalSciences，NanjingAgriculturalUniversity，Nanjing210095，China； Correspondingauthor，E mail：xiaorongfa”@njau．edu．cn) MA Cui，FAN Xiaorong，XU Guohua．ResponsesofriceplantsofWuyunjing7tonitrateasaffectedby0verexpressionof( NRrr7．2．ChinJRiceSci，20l1，25(4)：349—356． Abstract：A putative1ow—affinitynitratetransporter，os～R丁】．2 gene，wasidentifiedbasedonthericegenomicdatabaseand bioinformaticsanalysis．ByAgrobacteriumtumefacienstransformationwithaUbiquitin (Ubi)promoter，thegainoffunction ofriceOsNRT1．2wastestedbyover—expressioninWuyunjing7．TheOsNRT1．2eDNA from Knowledge—basedOryzaMo— lecularBiologicalEncyclopedia (K()M E)databaseencoded533aminoacidswith afull—lengthof2178bpandanopenreading fragment(ORF)of1732bp．TheexpressionpatternofOsNRT1．2 inWuyunjing7wasinvestigatedandtheresultsshowed thatitsmRNA wasonlyexpressedinrootsbutnotinshoots．Andtheover_exDressedplantsweregeneratedbythetransfor— mationofpUbi一 NRn ．2intoWuyunjing7．Wildtype(WT)andthreeTzgenerationtransgeniclines，namedas()E1，OE2 andOE8，wereusedinthegrowthexperimentat0．2and5．0mmol／Initratefor30daysandtheplantheightandrootlength wererecordedevery10 days．TheresultsshowedthattheplantheightofW T andtransgenicplantshadnodifferenceexcept OE8lineat5．0mmol／LNO3一，whiletheroot1engthoftransgeniclinessignificantlydecreasedatboth0．2and5．0mmol／I nitrate．Nitrogen(N)contentinshootsandrootswasnotdifferentbetweenWTandtransgeniclinesat0．2mmol／INO3 ． Butat5．0mmol／LNO3一，comparedwithWT，theshootnitrogencontentofOE2andOE8exceptOE1wasin