脑源性神经营养因子对持续惊厥后海马凋亡调控基因表达的影响①.pdfVIP

中国康复理论与实践2013 年1 月第19 卷第1 期 Chin J Rehabil Theory Pract, Jan. 2013, Vol. 19, No. 1 - 42 - DOI : 10.3969/j.issn. 1006-9771.2013.01.011 ·基础研究· 脑源性神经营养因子对持续惊厥后海马凋亡调控基因表达的影响① 1,2 2 1 2 苑爱云 ，蒋莉 ，侯梅 ，李欣 [摘要] 目的 观察外源性脑源性神经营养因子(BDNF)对惊厥持续状态(SC)后海马凋亡调控基因表达的影响。方法 选用成 年Wistar 鼠32 只制作氯化锂-匹罗卡品SC 模型，另外32 只为正常对照组。两组分别于一侧脑室内注射生理盐水、BDNF 、抗BDNF 抗体或不注射，于注射后6 h 处死。免疫组化观察海马Bcl-2 和c-Jun 蛋白的表达；原位杂交和RT-PCR 测定bcl-2 和c-jun mRNA 的 表达。结果 正常对照组双侧海马bcl-2 及c-jun 表达与同组无注射组比较无显著性差异。SC 组注射侧海马bcl-2 表达由高而低依次 为：SC-BDNF 组SC-无注射组=SC-NS 组(P0.05)SC-抗BDNF 组(P0.05) ，c-jun 表达则呈相反变化趋势(P0.05) ；SC 组注射对侧 海马bcl-2 及c-jun 表达与无注射对照组相比无显著性差异。结论 SC 后，脑室内注射外源性BDNF 可能通过上调凋亡抑制基因 bcl-2 、下调凋亡促进基因c-jun 表达发挥其保护作用。脑室内注射BDNF 在脑内扩散有限，在临床的应用受限。 [关键词] 惊厥持续状态；脑源性神经营养因子；凋亡；bcl-2 ；c-jun ；大鼠 Effects of Brain-derived Neurotrophic Factor on Apoptotic Regulated Genes in Hippocampal Cells after Status Convulsivus YUAN Ai-Yun, JIANG Li, HOU Mei, et al. Department of Neurology Rehabilitation, Qingdao Women Children Medical Healthacre Center, Qingdao 266034, Shandong, China Abstract: Objective To explore the effects of brain-derived neurotrophic factor (BDNF) on expression of apoptotic regulated genes (bcl-2 and c-jun) in hippocampus after status convulsivus (SC). Methods Seizures were induced in 32 adult Wistar rats with lithium-pilocar- pine intraperitoneal injection (SC), the other 32 rats were as the normal controls (NC). The rats were sacrificed 6 h after injection of normal saline (NS), BDNF, or anti-BDNF in left lateral ventricle (or no injection). The expression of Bcl-2 and c-Jun protein and bcl-2 and c-jun mRNA were investigated with immunocytochemistry, RT-PCR and in situ hybridization. Results The expression of bcl-