根据生物学变异度确定血液学检测下限的研究.pdfVIP

中国科技论文在线 根据生物学变异度确定血液学检测下限的 研究# 吴俊，赵洁，宋英，赵磊，赵京忠* 5 （北京大学人民医院检验科，北京 100044 ） 摘要：目的确定血常规分析，血小板聚集检验最低检测下限范围 方法 Sysmex2100 血液分 析仪检测血常规，将标本倍比稀释后重复检测 10 次样本，计算变异系数CV 值，做曲线及 计算方程，以1/2 生物变异度规定为允许CV 值，该CV 值代入方程为检测低限。对于血小 板聚集，稀释后检测血小板聚集，在低于平台区的范围定位检测下限。结果 白细胞检测下 10 限为0.11*109/l ，红细胞检测下限为0.14*1012/l ，红细胞压积检测下限3.03% ，血小板检测 下限为15.31*109/l。血小板聚集需要最低的血小板数量为200*109/l。结论 以生物变异度为 基础的CV 来确认仪器检测下限，方法可行，且与临床实际情况相符合。可作为确定仪器检 测下限的方法。。 关键词：血常规分析；血小板聚集；检测下限；生物变异度 15 Use biological variation to value the lower detect limit of hematology and platelet aggregation test WU Jun, ZHAO Jie, SONG Ying, ZHAO Lei, ZHAO Jingzhong (Department of laboratory medicine,Peoples hospital, Peking University, Beijing 100044) 20 Abstract: Objective To detect the lower limit of hematology, platelet aggregation. Methods: we use sysmex 2100 hematology analyzer blood cell account; Chrono-log platelet function instrument test platelet aggregation rate. After diluting samples, we retest the sample 10 times, plot X-axis with concentration, Y-axis with CV%, calculated the equation . We set 1/2 biological variation as standard CV to get the lower limit of detection. As for platelet aggregation, the 25 concentration below the plateau is the lower limit concentration. Results: Low limit detection of white blood cell, red blood cell, hematocrit, platelet is 0.11*109/l, 0.14*1012/l, 3.03%, 15.31*109/l, respectivly. The low concentration of platelet aggregation is 200*109/l. Conclus