高效液相色谱法检测水产品中的孔雀石绿和结晶紫.pdfVIP

第 29 卷 第 1 期 广东海洋大学学报 Vol.29 No.1 2009 年 2 月 Journal of Guangdong Ocean University Feb. 2009 高效液相色谱法检测水产品中的孔雀石绿和结晶紫 吴仕辉，朱新平，郑光明，陈昆慈，戴晓欣，潘德博 （中国水产科学研究院珠江水产研究所，广东 广州，510380) 摘 要：样品中的孔雀石绿、结晶紫经试剂盒提取，浓缩后用经固相萃取柱净化、硼氢化钾还原、反向色谱柱分 离，使用荧光检测器检测，孔雀石绿、结晶紫的加标回收率在 76.1 % ～92.5 %之间，相对标准偏差 1.9 % ～5.8 % ， 检出限均为 0.5 µg/kg。结果表明：该方法检测孔雀石绿（MG ）、结晶紫（GV ）处理简单，灵敏度高，节省时间， 可用于大量样品的快速分析。 关键词：水产品；孔雀石绿；结晶紫；检测；高效液相色谱法（HPLC ） 中图分类号：S948 文献标志码：A 文章编号：1673-9159 (2009)01-0054-04 Determination of Malachite Green and Gentian Violet Residues in Fishery Products by High Performance Liquid Chromatography(HPLC) with Fluorescence Detector WU Shi-hui, ZHU Xin-ping, ZHENG Guang-ming, CHEN Kun-ci, DAI Xiao-xin, PAN De-bo (Pearl River Fishery Research Institute, Chinese Academic of Fishery Science, Guangzhou, 510380 China) Abstract: Malachite green and gentian violet residues in fishery products were determined by high performance liquid chromatography method coupled with fluorescence detector. Samples were extracted by pre-treatment kit for extraction of MG, LMG, GV, LGV and purified by solid-phase extraction cartridges. After concentrated, samples were deoxidized by potassium borohydride. The MG and GV were detected with FLD detector after C18 column separation. The recoveries for fortified fish tissue were 76.1 % ～92.5 %, The relative standard deviation(RSD) values for repeatability were 1.9 % ～5.8 % ．The detection limit for MG and CV was 0.5 µg/kg, respectively. Key words: fisher product ；malachite green ；