筛选与拟南芥绒毡层发育过程中重要的转录因子DYT1相互作用的调控蛋白因子.pdfVIP

第40卷第2期 上海师范大学学报 (自然科学版) Vo1．40．No．2 2011年 4月 JournalofShanghaiNormalUniversity(NaturalSciences) Apr．，201l SUNLi—cheng，WEIJia—li，CHUYan-xia，ZHOUShu—min， YUANXiao-jun，ZHANGWei (ShanghaiKeyLaboratoryofBio-energyCrops，ShanghaiUniversity，Shanghai200444，China) Abstract：DYT1isacoretranscriptionfactorforArabidopsistapetum development．Impairmentof DYT1functioncausesfailureofmosttapetum—relatedfunctions．However，DYT1aloneisnotsuffi— cientofroveralltapetum function，suggestingthereshouldbeotherfactorsDYT1workswith．Using DYT1asbaitinyeasttwo—hybridsystem，screeningofrtheproteininteractingwith DYT1wasper- formedinthisreport．20positivecolonieswereobtained．Blastsequencecomparisonacquired8can— didatesequences，from which6candidateswereexhibitedtoencodeputativetranscriptionfactorsor othertranscription—regulatingfactors．5oftheseregulatorygeneswereconfimr edtobeexpressedin anther．Furthermorewefoundanotherantherindispensable transcriptionfactorAMS couldinteract withDYT1in yeast，suggesting thatthesetwotranscription factorsofrm heterozygoustranscription complextocontroltheexpressionofdownstream genesinantherdevelopment． Keywords：Arabidopsis；tapetum；DYT1；AMS；yeasttwo-hybrid CLCnumber：Q37 Documentcode：A ArticleID：1000-5137(2011)02-0179-07 1 Introduction Thetapetum isanutrienttissueoftheantheroffloweringplants，essentialofrnomr almalereproduction． Th eimpaimr entsoftapetum oftenleadtomalesterility．Uptodate，several geneshavebeenidentifiedinthe investigationtothemolecularmachinery ofmalesterilityofthemodelplantArabidopsisthaliana．Amongthem DYT1showstobeprominentsinceitissofartheearliestexpressedgeneidentifiedtoencodeabHLH tran— scriptionfactor．2 1mutantexhibitsabnomralanthermorphologybeginningatantherstage5，withexcessand enlargedvacuolesint