出口原奶和奶粉中三聚氰胺的快速检测方法 英文意见稿.docVIP

Foreword This standard was drafted in accordance with the rule of GB/T 1.1-2009. Please note that some of the elements of this standard may involve patents, but the Standards Organization does not assume responsibility for identifying these patents. This standard consists of two methods:the first method is ELISA,the second method is GICA Please choose an appropriate method to detect that is based on the detected objects and limited requirements. Annex A,annex B and annex C of this are informative annexs. This standard was proposed by and is under the charged of Certification and Accreditation Administration of the People’s Republic of China. This standard was drafted by Beijing Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China. The main drafters of this standard are This part is a professional standard for entry-exit inspection and quarantine promulgated for the first time. Note：This English Version, a translation from the Chinese text, is solely for guidance.powdered milk for export Scope This standard specifies two methods for rapid detection of melamine in raw milk and powdered milk of export by enzyme linked immunosorbent assay （ELISA）and gold immunochromatography assay (GICA). This standard is applicable to the screen of melamine in raw milk and powdered milk. Cited normative documents Following documents are necessary for the application of this present file. Only the version dated can be applied to the present documents, if not, the latest edition can do (including all the modified form). GB／T 6682 Water for analytical laboratory use - Specification and test methods (neq ISO 3696:1987) The First Method ELISA Principle This detection method is based on competitive colorimetric ELISA assay. The Free melamine in the testesd samples and coated antigen compete for the enzyme-labled antiboday in microtiter plates, than add in enzyme-labeled secondary antibody solution, according to the color reaction of substrate induced by the enzy