银杏叶提取物对酒精性脂肪肝大鼠肝脏的保护作用及其作用机.docVIP

银杏叶提取物【摘要】目的：观察银杏叶提取物对酒精性脂肪肝大鼠肝脏的保护作用，并探讨其作用机制。方法：50只大鼠随机分为5组(对照组，模型组和低、中、高浓度药物治疗组)，每组各10只。A组为正常对照组，不做特殊处理，B、C、D、E四组制备酒精性脂肪肝大鼠模型，同时C、D、E三组给予银杏叶提取物治疗(50mg/kg、100mg/kg、200mg/kg)；检测5组大鼠血清ALT、AST、γ-GT、Leptin含量及肝脏组织GSH-PX、SOD、MDA、GSH、Leptin蛋白的表达情况。结果：治疗组血清ALT、AST、γ-GT和肝组织MDA等指标较模型组明显降低(P0.05)且随治疗药物浓度上升，各指标下降更显著(P0.05)；治疗组GSH、SOD和GSH-PX等指标水平较模型组明显升高(P0.05)，且随治疗药物浓度上升，各指标升高更显著(P0.05)；治疗组血清和肝组织Leptin表达水平较模型组明显降低(P0.05)且随治疗药物浓度上升，各指标下降更显著(P0.05)。结论：银杏叶提取物通过抑制Leptin表达而起到保护酒精性脂肪肝大鼠肝脏的作用【关键词】银杏叶提取物；酒精性脂肪肝；瘦素 The protect role of ginkgo biloba extract for the liver of rats with alcoholic fatty and its mechanism research [Abstract] Objective: To observe he protect role of ginkgo biloba extract for the liver of rats with alcoholic fatty, and explore the mechanism. Methods: Fifty rats were divided into 5 groups (control group, model group and low, middle, high dose treatment group), every group was 10 rats. A group was normal control group, with no special treatment, B, C, D, E groups were made to rat liver fibrosis model. At the same time, C, D, E groups were treated with Ginkgo biloba extract(50mg/kg, 100mg/kg, 200mg/kg). The expression of ALT, AST, γ-GT, Leptin in serum and GSH-PX, SOD, MDA, GSH, Leptin were detected. Results: The expression of ALT, AST, γ-GT, MDA in treatment group were significantly lower than that of model group (P0.05), and with the therapeutic drug concentration rises, each index decreased more obviously(P0.05). The GSH, SOD and GSH-PX levels in treatment group were significantly higher than that of model group (P0.05), and with the therapeutic drug concentration rises, each index increased more significantly (P0.05). The leptin expression levels of treatment group in serum and liver tissue were significantly reduced than that of the model group(P0.05), and with the therapeutic drug concentration rises, each index decreased more obviously(P0.05). Conclusion: Ginkgo biloba extract could protect the liver function of alcoholic fatty liver rat through the inhi