一株苯酚降解菌的分离鉴定及响应面法优化其固定化.pdfVIP

中国环境科学 2014,34(2) ：518~525 China Environmental Science 一株苯酚降解菌的分离鉴定及响应面法优化其固定化 * 葛启隆,岳秀萍 ,王国英 (太原理工大学环境科学与工程学院, 山西 太原 030024) 摘要：从太原市焦化厂废水活性污泥中分离、筛选出一株苯酚降解细菌,经生理生化反应和 16S rRNA 鉴定,该菌株为Diaphorobacter 属细 菌,命名为 PD-07.代谢机制研究表明,苯酚可诱导该菌合成邻苯二酚 2,3-加氧酶降解苯酚.为了提高该菌株对苯酚的降解率, 以海藻酸钙为 材料,对该菌株进行包埋固定化研究.首先采用Plackett–Burman 实验设计筛选出影响固定化菌株苯酚降解率的关键因素,然后采用最陡爬坡 实验逼近最大苯酚降解率响应区域.最后用 Box–Behnken 实验设计及响应面回归分析,应用二次方程对实验数据进行拟合得,拟合曲线与实 验实测值相关性良好,最佳条件为海藻酸钠浓度 3.83%(m/V) 、CaCl2 0.3mol/L 、菌胶比 1:26.73、固定化时间2h、摇床转速 180r/min、培养 温度 30℃、初始pH 值 7.2、液固比4.86:1,在此条件下苯酚降解率可达 96.89%. 关键词：Diaphorobacter sp.PD-07 ；Plackett–Burman ；海藻酸钠；固定化细胞；苯酚；生物降解；响应面法 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2014)02-0518-08 Isolation and identification of a phenol-degrading strain and optimization for its immobilization using response * surface methodology. GE Qi-long, YUE Xiu-ping , WANG Guo-ying (College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China). China Environmental Science, 2014,34(2)：518~525 Abstract ：A phenol-degrading strain was isolated from the activated sludge of a cokes wastewater treatment facility in Taiyuan City. Based on its physiology, biochemical characters and the analysis of its 16S rRNA gene sequence, the isolated strain named PD-07 was identified as a member of the genus Diaphorobacter . Metabolic pathway research showed that the strain could be induced to synthesize intracellular catechol-2,3-dioxygenase.To improve the phenol degradation rate, a study of embedding immobilized strain was conducted with calcium alginate as material. Firstly, factors playing important roles in the phenol degradation rate of immobilized cell were selected based on Plackett-Burman design. Then the path of steepest ascent was undertaken to approach the optimal region of the phen