重复性“饥饿-再投喂”大鼠体重控制与能量平衡调节的初步研究.DOCVIP

临床医学论文-重复性“饥饿/再投喂”大鼠体重控制与能量平衡调节的初步研究 【摘要】? 目的:经过重复性“饥饿/再投喂”摄食方式的处理，探讨大鼠有关体重调节的瘦素（leptin）、生长激素（GH）和甲状腺激素（T4）与肥胖发生机制的关系。方法:设计“重复性饥饿1 d/再投喂1 d，持续6 w”的饲喂（基础饲料和高脂饲料）处理动物模型，用放射免疫的方法测定血清中leptin、GH、T4的水平。结果:幼年大鼠血清leptin含量相对比成体低，隔天饲喂基础饲料组和高脂饲料组中大鼠体重减轻，而leptin水平明显高于对照组，且与体重明显呈负相关；幼年处理组GH比对照组略高，但成年大鼠处理组随着体重减轻，GH水平却有下降；幼年和成年处理组T4水平都有增加。结论:Leptin与神经内分泌系统组成一个双向闭合环路，leptin与GH、T4共同作用于下丘脑发挥体重调节功能。 【关键词】? 能量平衡 大鼠 瘦素 甲状腺激素 生长激素 饥饿 再投喂 ??? A primary study on controlling body weight and energy balance in repeated fasting/refeeding rats?LIU Chong-bin, SHENTU Yang-ping, ZHANG Yu-Qing, et al. Department of Physiology, Wenzhou Medical College, Wenzhou 3250组35 牋?Abstract:?Objective:To explore the relationship among controlling body weight, energy balance and mechanisms of obesity associated with leptin, GH and T4 after repeating fasting/refeeding rats. Methods: A model of repeating fasting/refeeding rats (common feed and high fat feed) was designed, using irradiation immunity to determine the contents of leptin, GH and T4. Results: The level of leptin of young rats was lower than that of adult rats, the body weight of rats after repeating fasting/refeeding was lighter than that of the controls, but the level of leptin of serum was higher than that of the controls and it was obvious negative correlated with body weight. The growth hormone of serum was slightly higher in young rats than that in the contral group. but decreasing with the lowering of body weight in adult rats. The contents of T4 increaseed in young and adult rats. Conclusion: Two closed loop Leptin and neuroendocrinological system make up of a two-direction closed loop. Leptin, GH and T4 together effect on hypothalamus to play its functions for control body weight.?牋?Key words:?energy balance; rat; leptin; GH; T4; repeating fasting/refeeding ??? 动物及人类的身体能量平衡与体重控制是环境因素和遗传因素相互作用的结果[1，2]。关于哺乳动物及人类的身体能量平衡调节，目前认为脂肪细胞分泌的信号分子瘦素（leptin）经循环系统运输到下丘脑发挥作用，通过降低食欲、提高交感神经系统兴奋性而控制体重[3，4]。后来发现肥胖人体内leptin往往还保持较高水平，说明该激素可能失去了对体重增长的调节能力，即产生