尼卡地平对沙土鼠脑缺血后细胞凋亡的影响.pdfVIP

尼卡地平对沙土鼠脑缺血后细胞凋亡的影响 中文摘要 刁 勺 又 J r 夕 \ 中文摘要 目的:研究沙土鼠短暂性完全性前脑缺血后细胞凋亡及尼卡地平处理 后的影响。方法:阻断沙土鼠双侧颈总动脉巧分钟造成前脑缺血模型。 实验动物被随机分为假手术组、缺血再灌注组、尼卡地平缺血前处理 组，尼卡地平缺血后处理组。用TdT介导的原位末端标记 (TUNED法 来检测死亡神经元的DNA片段。HE染色计数海马CAI区Imm长度内正 常的锥体细胞数:令果:(1)与假手术组比较，缺血再灌注后2-4天， 海马CAI区正常锥体细胞数明显减少 (P0.01)(2)缺血再灌注后2-4 天，尼卡地平处理组海马CAI区正常锥体细胞数明显比未处理组多 (P0.01)。(3)尼卡地平处理组明显减少缺血后海马、皮质细胞凋亡 (P0.01扫结论，完全性前脑缺血后存在细胞凋亡;尼卡地平可抑制 缺血后海马和皮质的细胞凋亡。 关键词:脑缺血，短暂性;细胞凋亡;尼卡地平;沙土鼠 尼卡地平对沙土鼠脑缺血后细胞凋亡的影wA — 一一一一一一一一一一一进翌鲤 Abstract Objective:Toexploretheneuronalapoptosisandeffectofnicardipine aftertransientforebrainischemiaingerbils.Methods:Transientforebrain ischemiawasinducedby15minutesbilateralcarotidoccusion.Allgerbils wererandomlydividedintoshamoperationgroup,ischemia-reperfusion group,nicardipinegroupbeforeischemia,nicardipinegroupafter ischemia,.FragmentedDNAwasidentifiedbyterminaldeoxynucleotidyl transferase(TdT)-mediateddUTP-biotinnick-labeling(TUNEL).The numbersofnormalpyramidalcellsinthehippocampusCAIfieldinImm lengthwerecountedwithhematoxylinandeosin.Results:(1)Compared withshamoperation,2to4daysafterischemia-reperfusion,thenormal pyramidalcellsinthehippocampusCAIfieldwerereducedin ischemia-reperfusion group(P0.01).(2) 2 to 4 days after ishemia-reperfusion，thenormalpyramidalcellsinthehippocampusCAI field in nicardipine group were increased than those in ischemia-reperfusion group(P0.01).(3)Nicardipine significantly decreasedthenumberofapoptoticcellsinthehippocampusCAIfield andcerebralcortexafterishemia-reperfusion(p0.01).Conclusions:(1) Transientforebrainischemiamarkedlyproducedapoptoticcellincerebral cortexandhippocampusCAIpyramidalneurons.(2)Nicardipinehas inhibitory effectonischemia-inducedapoptosisofhippocampusand cerebralcortex. Keywords:transientcerebralischemia;apoptosis;nicard ;gerbil