重寄生过程中哈茨木霉转化菌株大量表达胞外碱性蛋白酶.docVIP

Trichoderma harzianum transformant has high extracellular alkaline proteinase expression during specific mycoparasitic interactions ?Genetics and Molecular BiologyPrint?ISSN?1415-4757 Genet. Mol. Biol.?vol. 21?n. 3?S?o Paulo?Sept.?1998 Maria Helena S. Goldman1 and Gustavo H. Goldman2 1Departamento de Biologia,Faculdade de Filosofia, Ciências e Letras de Ribeir?o Preto and 2Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeir?o Preto, USP, Av. do Café s/n, 14040-903 Ribeir?o Preto, SP, Brasil. Fax: 55-016-6331092. E-mail: ggoldman@usp.br? Send correspondence to G.H.G. ? ? ABSTRACT The mycoparasite Trichoderma harzianum produces an alkaline proteinase that may be specifically involved in mycoparasitism. We have constructed transformant strains of this fungus that overexpress this alkaline proteinase. Some of the transformants were assessed for alkaline proteinase activity, and those with higher activity than the wild type were selected for further studies. One of these transformant strains produced an elevated and constitutive pbr1 mRNA level during mycoparasitic interactions with Rhizoctonia solani. INTRODUCTION The mycoparasite Trichoderma harzianum may prove to be an effective control agent for many phytopathogenic fungi. It has been proposed that its mycoparasitic interaction proceeds in three major steps (Goldman et al., 1994a,b; Haran et al., 1996). Initially, the mycoparasite hyphae grow toward the host hyphae (Chet et al., 1981). Then the parasite attaches to the target hyphae, presumably mediated by a host lectin, and appressoria-like structures coil around the attacked cells (Elad et al., 1983a,b; Barak et al., 1985). Concurrently, degradation of ?(1,3)-glucans and chitin from the host cell wall has been observed (Elad et al., 1983b). Probably both mechanical pressure and cell wall degradation by hydrolytic enzymes are involved. Finally, the mycoparasite penetrates and/or lyses the host hyphae (Chet et al., 1981) and