乳腺癌细胞核DNA含量不同分析方法的比较.pdfVIP

中国体视学与图像分析 2003 年9 月 第8 卷 第3 期 154 CHINESE JOURNAL OF STEREOLOGY AND IMAGE ANALYSIS Vol.8 No.3 Sep.2003 文章编号：1007-1482（2003）03-0154-04 乳腺癌细胞核DNA含量不同分析方法的比较 李志华，夏潮涌，黄中新，覃莉，唐海兰，饶晓黎 （暨南大学医学院组织胚胎学教研室广州 510632 ） 【摘要】目的：比较组织原位分析乳腺癌细胞核DNA含量的不同方法。方法：收集30例乳腺癌 标本的归档蜡块，4µm和相邻8µm切片各一张，Feulgen染色，采用TIGER细胞图像分析仪测 量每个癌巢的以单个完整细胞核体积为单位计算的DNA指数和以细胞核切面面积为单位计算的 DNA指数，它们均以同一病例正常上皮细胞核作内参照。结果：（1）同一病例的VIOD分布直方 图比IOD更集中；（2）30例标本的90个样本中，以VIOD为单位所测得的DI值比以 IOD为单 位测得的DI值高。结论：使用细胞图像分析仪对组织切片内细胞核DNA含量进行定量分析时， 应以单个完整细胞核体积的DNA含量为单位，计算DI值。 【关键词】乳腺癌；图像分析；DI；切片厚度 【中图分类号】R318.51 【文献标识码】B COMPARE THE DIFFERENCE ON MEASURING METHODS OF DNA CONTENT OF BREAST CANCER LI Zhi-hua ，XIA Chao-yong ，HUANG Zhong-xin，QIN Li ，TANG Hai-lan，RAO Xiao-li (Department of Histology and Embryology，Medical College of Jinan University，Guangzhou，510632) 【ABSTRACT 】 Objective: To compare the difference of two methods on measuring DNA content of breast cancer；Methods : Thirty paraffin blocks of breast cancers were collected and cut on 4µm and 8µm adjacent section. The sections were stained in Feulgen. The two DI ( in volume unit of whole nuclei and in area unit of nuclei thin sections) of 3 cancer nests of every case were evaluated by TIGER cell image analyzer. The normal epithelial nuclei were controlled as interior reference. Result: (1) The histogram of VIOD is more concentrative than the one of AIOD in the same case. (2) The DI calculated by VIOD is higher than the one calculated by AIOD in the ninety samples of thirty cases. Conclusion: When DNA content on tumor tissue section were measured with cell image analyzer， DNA content in volume unit of whole nuclei should be obtained and DI was calculated. 【KEYWORDS 】 breast cancer ；im