高效液相色谱法测定食品中三氯蔗糖含量的研究.pdfVIP

# 156# 上海预防医学杂志 2005年第 17卷第 4期 S J PrevM ed, Vol 17, No. 4 : 1004 - 9231( 2005) 04 - 0156- 03 # # 高效液相色谱法测定食品中三氯蔗糖含量的研究 熊丽蓓, 戴承兵, 何倩琼 (上海市疾病预防控制中心, 上海 200336) : [目的] 研究食品中三氯蔗糖含量的分析方法 [ 方法] 利用高效液 色谱仪检测食品中三氯蔗糖的 含量 [ 结果] 建立了食品中三氯蔗糖的提取富集和净化等前处理方法, 采用高效液 色谱法蒸发光散射检测器测 定 [ 结论] 本方法能满足食品中三氯蔗糖含量的分析要求, 是一种简单可靠易于推广的检测方法 + : 三氯蔗糖; 高效液相色谱; 蒸发光散射 测器; 固相萃取 : R 122. 1 1: A M ethod for quan titat ive determ ination of sucralose in food XION L i- bei, DA I Cheng - bing, H E Q ian- qiong ( Shanghai Centerf or D isease Control and P revention, Shanghai 200336, China A bstrac t: [ O bj ective] To study a m et od for quantitative determ ination of sucralose in food. [M e thods] H ig per- form ance liqu id c rom atograp yw as used for quan titative determ ination of sucralose in foods. [ Resu lts] Sam ple pro- treat- m ent procedures included sucralose extraction, enric m ent and purification . A ig perform ance liqu id c rom atograp y gradient m et od w it an evaporative lig t- scattering detector as been developed for t e determ ination of sucralose. T e average recovery ratew as 94. 3% and t e relative standard deviation w as 2. 40% . [ Con clusion] T is m et od can m eet t e analysis requ ire- m ent for determ ination of sucralose in food and it is a s mi p le, reliable met od easy for popu larizing. K ey w ord s: Sucralose; H ig perform ance liqu id c rom atograp y; Evaporative lig t- scattering detector; Solid p ase extraction [ 1] , Sucralose, (2g) 1970 TATE LYLE , (TATE LYLE , \99. 5% ) 1. 2 色谱条件 , 600, AD I